Department of Histology and Embryology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Sıtkı Koçman University, Muğla, Turkey.
Biotech Histochem. 2022 Feb;97(2):152-157. doi: 10.1080/10520295.2021.1918766. Epub 2021 Apr 28.
Oxygen radicals participate in the pathogenesis of heart damage. Diabetes accelerates the formation of reactive oxygen species (ROS). We investigated the effects of the antioxidants, melatonin, quercetin and resveratrol, on cardiomyopathy and apoptosis in rats with streptozotocin (STZ) induced diabetes mellitus (DM). Rats were divided into five groups of seven: control, DM, DM + melatonin, DM + quercetin and DM + resveratrol. All treatments were begun with a single dose of STZ to induce diabetes and experimental treatments were continued daily for 30 days. Morphologic and apoptotic changes were analyzed by histological assessment. The heart tissue of the control group exhibited normal histology, whereas the heart tissue of the DM group exhibited vacuolization, necrosis, congestion, infiltration and myofibril loss. The DM group exhibited significantly increased apoptosis compared to the control group. Differences in anti-apoptotic effects were statistically significant for all three antioxidant treatment groups; the anti-apoptotic effects of quercetin and resveratrol were similar. Melatonin, resveratrol and quercetin exhibited protective effects against diabetic heart damage.
氧自由基参与了心脏损伤的发病机制。糖尿病加速了活性氧（ROS）的形成。我们研究了抗氧化剂褪黑素、槲皮素和白藜芦醇对链脲佐菌素（STZ）诱导的糖尿病大鼠心肌病和细胞凋亡的影响。大鼠分为五组，每组 7 只：对照组、糖尿病组、糖尿病+褪黑素组、糖尿病+槲皮素组和糖尿病+白藜芦醇组。所有治疗均采用单次 STZ 注射诱导糖尿病，实验治疗持续 30 天。通过组织学评估分析形态和凋亡变化。对照组的心脏组织表现出正常的组织学，而糖尿病组的心脏组织表现出空泡化、坏死、充血、浸润和肌原纤维丧失。与对照组相比，糖尿病组的细胞凋亡明显增加。所有三种抗氧化剂治疗组的抗凋亡作用差异均具有统计学意义；槲皮素和白藜芦醇的抗凋亡作用相似。褪黑素、白藜芦醇和槲皮素对糖尿病性心脏损伤具有保护作用。
