Division of Pediatric Neurology, Department of Neurology, Johns Hopkins University School of Medicine, MD 21205.
Department of Pediatrics, Johns Hopkins University School of Medicine, MD 21205.
eNeuro. 2021 May 21;8(3). doi: 10.1523/ENEURO.0015-21.2021. Print 2021 May-Jun.
AMPA receptor (AMPAR) mobility within synapses has been extensively studied However, whether similar mobility properties apply to AMPARs has yet to be determined. Here, we use two-photon fluorescence recovery after photobleaching (FRAP) to study AMPAR mobility within individual dendritic spines in live animals using an overexpression vector. We demonstrate the existence of mobile and immobile fractions of AMPARs across multiple cortical regions and layers. Additionally, we find that AMPAR mobility can be altered in response to administration of corticosterone, a condition that mimics exposure to stress.
AMPA 受体 (AMPAR) 在突触内的流动性已被广泛研究。然而，类似的流动性特性是否适用于 AMPAR 仍有待确定。在这里，我们使用双光子荧光漂白后恢复 (FRAP) 技术，使用过表达载体在活体动物的单个树突棘内研究 AMPAR 的流动性。我们证明了在多个皮质区域和层中存在 AMPAR 的可动和不可动部分。此外，我们发现 AMPAR 的流动性可以响应皮质酮的给药而改变，皮质酮是一种模拟应激暴露的条件。
