Bland B H, Colom L V, Konopacki J, Roth S H
Department of Psychology, University of Calgary, Alta., Canada.
Brain Res. 1988 May 3;447(2):364-8. doi: 10.1016/0006-8993(88)91141-9.
Intracellular recordings were made in the CA1, CA3 and dentate cell layers prior to, during and after the bath perfusion of 50 microM carbachol on hippocampal slices. Fifty-six percent of the cells in this sample were termed theta (theta)-related, i.e., they exhibited membrane potential oscillations of 5-28 mV and rhythmic spike discharges related to the carbachol-induced extracellular theta-rhythm. The remaining 44% of the cells did not show the above relationships to the extracellular theta-rhythm. Carbachol produced an overall depolarization in all cells, in the range of 10-20 mV. These results demonstrated the cellular basis of carbachol-induced theta in hippocampal slices. This preparation will be a valuable model for studying cellular mechanisms and network properties underlying electroencephalographic activity.
在向海马切片浴灌流50微摩尔卡巴胆碱之前、期间和之后，对CA1、CA3和齿状细胞层进行了细胞内记录。该样本中56%的细胞被称为与θ波相关，即它们表现出5 - 28毫伏的膜电位振荡以及与卡巴胆碱诱导的细胞外θ节律相关的节律性锋电位发放。其余44%的细胞未表现出与细胞外θ节律的上述关系。卡巴胆碱在所有细胞中产生了10 - 20毫伏范围内的总体去极化。这些结果证明了海马切片中卡巴胆碱诱导的θ波的细胞基础。该制备方法将成为研究脑电图活动潜在的细胞机制和网络特性的有价值模型。
