Vallarino M, Danger J M, Fasolo A, Pelletier G, Saint-Pierre S, Vaudry H
Instituto di Anatomia Comparata, Università di Genova, Italy.
Brain Res. 1988 May 10;448(1):67-76. doi: 10.1016/0006-8993(88)91102-x.
Using a specific antiserum raised against synthetic neuropeptide Y (NPY), the distribution of immunoreactivity in the brain and pituitary of the elasmobranch fish Scyliorhinus canicula has been examined with the indirect fluorescence and the peroxidase-antiperoxidase methods. The highest density of NPY-immunoreactive neurons was found in the basal telencephalon and in the hypothalamus. Numerous NPY-containing perikarya were located in the entopeduncular and the preoptic nuclei, in the nucleus lobi lateralis and in the nucleus lateralis tuberis. NPY-immunopositive fibers were observed throughout the fish brain. In particular, dense networks of fibers were present in the entopeduncular and the habenular nuclei, in the nucleus tuberculi posterioris and in the lateral lobes. Scattered fibers were observed in all other parts of the brain except in the cerebellum where no NPY-immunoreactive material could be detected. A plexus of NPY-immunoreactive fibers arising from the preoptic neurosecretory complex appeared to run through the basal hypothalamus and the pituitary stalk. These fibers terminated in the intermediate lobe of the pituitary, suggesting that NPY may be involved in the control of melanotropin secretion. The NPY-immunoreactive material localized in the brain and pituitary was characterized by combining high-performance liquid chromatography (HPLC) analysis and radioimmunological detection. Brain and pituitary extracts showed a good cross-reactivity to the NPY antiserum, but serial dilutions of tissue samples did not completely parallel the standard curve. HPLC analysis resolved two major forms of immunoreactive NPY in the hypothalamus while the pars intermedia contained only authentic NPY. The widespread distribution of NPY neurons in the fish brain and pituitary suggests the involvement of NPY in a variety of physiological functions, including the neuroendocrine control of the pituitary.
利用针对合成神经肽Y（NPY）制备的特异性抗血清，采用间接荧光法和过氧化物酶-抗过氧化物酶法，研究了板鳃亚纲鱼类小斑猫鲨脑和垂体中免疫反应性的分布。NPY免疫反应性神经元密度最高的区域位于基底端脑和下丘脑。许多含NPY的神经细胞体位于内侧缰核、视前核、外侧叶核和外侧结节核。在鱼脑各处均观察到NPY免疫阳性纤维。特别是，在内侧缰核、缰核、后结节核和外侧叶中存在密集的纤维网络。除小脑未检测到NPY免疫反应性物质外，在脑的所有其他部位均观察到散在的纤维。来自视前神经分泌复合体的NPY免疫反应性纤维丛似乎贯穿基底下丘脑和垂体柄。这些纤维终止于垂体中间叶，提示NPY可能参与促黑素分泌的调控。通过高效液相色谱（HPLC）分析和放射免疫检测对脑和垂体中定位的NPY免疫反应性物质进行了表征。脑和垂体提取物与NPY抗血清表现出良好的交叉反应性，但组织样品的系列稀释并不完全与标准曲线平行。HPLC分析在小丘脑中解析出两种主要形式的免疫反应性NPY，而中间部仅含有天然NPY。NPY神经元在鱼脑和垂体中的广泛分布表明NPY参与多种生理功能，包括对垂体的神经内分泌控制。
