Department of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193, Aveiro, Portugal.
Department of Applied Chemistry, Chalmers University of Technology, Kemivägen 4, 412 96, Gothenburg, Sweden.
Sci Rep. 2021 Apr 27;11(1):9099. doi: 10.1038/s41598-021-88482-3.
The sustainable cellular delivery of the pleiotropic drug curcumin encounters drawbacks related to its fast autoxidation at the physiological pH, cytotoxicity of delivery vehicles and poor cellular uptake. A biomaterial compatible with curcumin and with the appropriate structure to allow the correct curcumin encapsulation considering its poor solubility in water, while maintaining its stability for a safe release was developed. In this work, the biomaterial developed started by the preparation of an oil-in-water nanoemulsion using with a cytocompatible copolymer (Pluronic F 127) coated with a positively charged protein (gelatin), designed as G-Cur-NE, to mitigate the cytotoxicity issue of curcumin. These G-Cur-NE showed excellent capacity to stabilize curcumin, to increase its bio-accessibility, while allowing to arrest its autoxidation during its successful application as an anticancer agent proved by the disintegration of MDA-MB-231 breast cancer cells as a proof of concept.
具有生物相容性的材料与适当的结构相结合，以允许在考虑到其在水中的低溶解度的情况下正确封装姜黄素，同时保持其稳定性以实现安全释放。在这项工作中，所开发的生物材料首先通过使用具有细胞相容性的共聚物（Pluronic F 127）制备油包水纳米乳液来制备，该共聚物（Pluronic F 127）涂覆有带正电荷的蛋白质（明胶），设计为 G-Cur-NE，以减轻姜黄素的细胞毒性问题。这些 G-Cur-NE 显示出出色的稳定姜黄素的能力，增加了其生物利用度，同时允许在成功用作抗癌剂时阻止其自氧化，这一点已通过 MDA-MB-231 乳腺癌细胞的崩解得到证实，这是一个概念验证。
