Columba-Palomares María C, Mariana Montiel-Ruiz Rosa, Corona Sánchez Lucia, Palafox-Gante Daniel, Rodríguez-López Verónica
Laboratory of Chemistry of Natural Products and Pharmacognosy, Faculty of Pharmacy, Autonomous University of the State of Morelos (UAEM). Morelos, México.
Laboratory of Pharmacology, Center of Biomedical Research of the South, Mexican Institute of Social Security (IMSS). Morelos, México.
Avicenna J Phytomed. 2021 Mar-Apr;11(2):101-108.
Some species of the genus have been used different illnesses associated with pain and inflammation. The aim of this work was to characterize the biological activities (anti-inflammatory and analgesic) and antioxidant capacity of methanol and dichloromethane extracts of .
In this study, topical anti-inflammatory activity was tested in an model of 12-O-tetradecanoylphorbol acetate (TPA) induced mouse ear edema of organic extracts (doses: 0.03, 0.1 and 0.3 mg/ear). The antinociceptive activity was assessed using the formalin test in mice of organic extracts (doses: 56, 100 and 300 mg/kg). Moreover, the antioxidant capacity of the extracts was determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azinobis (3-ethylbenzothiaziline-6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays.
Methanol (RcM) and dichloromethane (RcD) extracts of the aerial parts were found to inhibit ear edema (48.95 and 40.13%, respectively) at a dose of 0.3 mg/ear. Acute treatment with RcM produced a significant antinociceptive effect in the late phase of formalin-induced nociception. Moreover, RcM at doses of 56, 100 and 300 mg/kg showed a significant antinociceptive effect through the early and late phases in the formalin test. RcM and RcD showed weak antioxidant capacities in the ABTS and DPPH assays; however, when their reducing capacity was evaluated by the FRAP assay, RcM showed a reducing activity similar to standard at the proven concentration of 1000 μg/ml.
According to the experimental findings, the organic extracts of display remarkable antinociceptive and anti-inflammatory activities.
该属的某些物种已被用于治疗与疼痛和炎症相关的不同疾病。本研究的目的是表征[具体植物名称]地上部分的甲醇提取物和二氯甲烷提取物的生物活性（抗炎和镇痛）及抗氧化能力。
在本研究中，通过12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导的小鼠耳肿胀模型测试有机提取物（剂量：0.03、0.1和0.3mg/耳）的局部抗炎活性。使用福尔马林试验评估有机提取物（剂量：56、100和300mg/kg）对小鼠的抗伤害感受活性。此外，使用2,2 - 二苯基 - 1 - 苦基肼（DPPH）、2,2'- 偶氮二（3 - 乙基苯并噻唑啉 - 6 - 磺酸）（ABTS）和铁还原抗氧化能力（FRAP）测定法测定提取物的抗氧化能力。
[具体植物名称]地上部分的甲醇提取物（RcM）和二氯甲烷提取物（RcD）在剂量为0.3mg/耳时可抑制耳肿胀（分别为48.95%和40.13%）。RcM急性处理在福尔马林诱导的伤害感受后期产生显著的抗伤害感受作用。此外，在福尔马林试验中，56、100和300mg/kg剂量的RcM在早期和后期均显示出显著的抗伤害感受作用。RcM和RcD在ABTS和DPPH测定中显示出较弱的抗氧化能力；然而，当通过FRAP测定法评估其还原能力时，在1000μg/ml的验证浓度下，RcM显示出与标准品相似的还原活性。
根据实验结果，[具体植物名称]的有机提取物具有显著的抗伤害感受和抗炎活性。
