Wang Shu-Hua, Sun Zhe, Li Hui, Ye Xin-Jian, Feng Jian-Ying
School of Stomatology, Zhejiang Chinese Medical University. Hangzhou 310053, Zhejiang Province, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2021 Feb;30(1):17-22.
Low-magnitude, high-frequency（LMHF） loading has been confirmed to play an important role in bone healing. The present study aimed to evaluate the effect of LMHF loading applied directly to titanium dental implants on peri-implant bone healing and implant.
The mandibular premolars and molars were extracted from six male Beagle dogs. Three months post-extraction, six titanium implants (Aadva Standard Implant Narrow, 3.3 mm×8 mm) were inserted into the mandibular premolar and molar area (three implants per side) in each of the six dogs. In each animal, one side was randomly selected to undergo daily LMHF loading (experimental group), while the other side had no further intervention (control group). The loading was applied directly to the implant abutment using an individual jig and a custom-made loading device (8 μm,100 Hz). The implant stability quotient (ISQ) was tested every week. Three dogs were euthanized after 2 weeks, and three were euthanized after 8 weeks. Tissue samples were fixed and stained for micro-computed tomography(micro-CT) and histomorphometric analyses. The data were statistically analyzed using SPSS 13.0 software package.
The experimental group had significantly increased peri-implant bone volume relative to tissue volume in region of interest 2 (100-500 μm) compared with the control group after 2 weeks of loading (P<0.05); however, there was no significant difference between the two groups after 8 weeks (P>0.05). The ISQ value and the micro-CT results did not differ between the two groups during the study period.
LMHF loading positively influences peri-implant bone healing and osseointegration in the early healing period.
低强度、高频（LMHF）加载已被证实对骨愈合起重要作用。本研究旨在评估直接施加于钛牙种植体的LMHF加载对种植体周围骨愈合及种植体的影响。
从6只雄性比格犬下颌拔除前磨牙和磨牙。拔牙后3个月，在每只犬的下颌前磨牙和磨牙区（每侧3枚种植体）植入6枚钛种植体（Aadva标准窄种植体，3.3 mm×8 mm）。在每只动物中，随机选择一侧每天进行LMHF加载（实验组），而另一侧不进行进一步干预（对照组）。使用单独的夹具和定制加载装置（8μm，100Hz）将加载直接施加于种植体基台。每周测试种植体稳定性商数（ISQ）。2周后对3只犬实施安乐死，8周后对另外3只犬实施安乐死。将组织样本固定并染色，用于显微计算机断层扫描（micro-CT）和组织形态计量学分析。使用SPSS 13.0软件包对数据进行统计学分析。
加载2周后，实验组种植体周围感兴趣区域2（100 - 500μm）的骨体积相对于组织体积显著增加，与对照组相比差异有统计学意义（P<0.05）；然而，8周后两组之间无显著差异（P>0.05）。在研究期间，两组的ISQ值和micro-CT结果无差异。
LMHF加载在愈合早期对种植体周围骨愈合和骨整合有积极影响。
