[The effect of low-magnitude high-frequency loading on peri-implant bone healing and implant osseointegration in Beagle dogs].

PURPOSE

Low-magnitude, high-frequency（LMHF） loading has been confirmed to play an important role in bone healing. The present study aimed to evaluate the effect of LMHF loading applied directly to titanium dental implants on peri-implant bone healing and implant.

METHODS

The mandibular premolars and molars were extracted from six male Beagle dogs. Three months post-extraction, six titanium implants (Aadva Standard Implant Narrow, 3.3 mm×8 mm) were inserted into the mandibular premolar and molar area (three implants per side) in each of the six dogs. In each animal, one side was randomly selected to undergo daily LMHF loading (experimental group), while the other side had no further intervention (control group). The loading was applied directly to the implant abutment using an individual jig and a custom-made loading device (8 μm,100 Hz). The implant stability quotient (ISQ) was tested every week. Three dogs were euthanized after 2 weeks, and three were euthanized after 8 weeks. Tissue samples were fixed and stained for micro-computed tomography(micro-CT) and histomorphometric analyses. The data were statistically analyzed using SPSS 13.0 software package.

RESULTS

The experimental group had significantly increased peri-implant bone volume relative to tissue volume in region of interest 2 (100-500 μm) compared with the control group after 2 weeks of loading (P<0.05); however, there was no significant difference between the two groups after 8 weeks (P>0.05). The ISQ value and the micro-CT results did not differ between the two groups during the study period.

CONCLUSIONS

LMHF loading positively influences peri-implant bone healing and osseointegration in the early healing period.