Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan; School of Stomatology, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan.
J Prosthodont Res. 2018 Oct;62(4):497-502. doi: 10.1016/j.jpor.2018.07.004. Epub 2018 Aug 20.
Low-magnitude, high-frequency (LMHF) loading plays an important role in bone healing. The present study aimed to evaluate the effect of LMHF loading applied directly to titanium dental implants on peri-implant bone healing and implant osseointegration.
The mandibular premolars and molars were extracted from six male Beagle dogs. Three months post-extraction, each of the six dogs had three titanium implants (Aadva Standard Implant Narrow, Φ3.3×8mm) inserted into the mandibular premolar and molar area (three implants per side). In each animal, one side was randomly selected to undergo daily LMHF loading (treatment group), while the other side had no further intervention (control). The loading was applied directly to the implant abutment using an individual jig and a custom-made loading device (8μm, 100Hz). The implant stability quotient (ISQ) was tested every week. Three dogs were euthanized after 2 weeks, and three were euthanized after 8 weeks. Tissue samples were fixed and stained for micro-computed tomography (micro-CT) and histomorphometric analyses. Data were analyzed statistically, with significance set at p<0.05.
The treatment group had significantly increased peri-implant bone volume relative to tissue volume in region of interest 2 (100-500μm) compared with the control group after 2 weeks of loading (p<0.05); however, there was no significant difference between groups after 8 weeks. The ISQ value and the micro-CT results did not differ between groups during the study period.
LMHF loading positively influenced peri-implant bone healing in the early healing period.
低幅度、高频率（LMHF）加载在骨愈合中起着重要作用。本研究旨在评估直接施加于钛牙科种植体的 LMHF 加载对种植体周围骨愈合和种植体骨整合的影响。
从 6 只雄性比格犬中取出下颌前磨牙和磨牙。拔牙后 3 个月，每只狗的下颌前磨牙和磨牙区域植入 3 个钛种植体（Aadva Standard Implant Narrow，Φ3.3×8mm）（每侧 3 个种植体）。在每只动物中，随机选择一侧进行每日 LMHF 加载（治疗组），而另一侧则不进行进一步干预（对照组）。使用单独的夹具和定制的加载装置（8μm，100Hz）直接将加载施加于种植体基台。每周测试种植体稳定性指数（ISQ）。3 只犬在 2 周后处死，3 只犬在 8 周后处死。对组织样本进行固定和染色，用于微计算机断层扫描（micro-CT）和组织形态计量学分析。对数据进行统计学分析，显著性水平设为 p<0.05。
与对照组相比，治疗组在加载后 2 周时，感兴趣区域 2（100-500μm）的种植体周围骨体积相对于组织体积显著增加（p<0.05）；然而，在 8 周后，两组之间没有显著差异。在研究期间，两组之间的 ISQ 值和 micro-CT 结果没有差异。
LMHF 加载在早期愈合期对种植体周围骨愈合有积极影响。
