Department of biochemistry and nutrition, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, 11757, Egypt.
Egyptian Ministry of Environment, Cairo, 11728, Egypt.
Environ Sci Pollut Res Int. 2021 Sep;28(35):48517-48534. doi: 10.1007/s11356-021-14089-w. Epub 2021 Apr 28.
This research was intended to evaluate the antidiabetic effect of single or combined administration of nanoparticles of zinc oxide nanoparticles (ZnONPs), chromium oxide nanoparticles (CrONPs), and selenium nanoparticles (SeNPs), on genetic and metabolic insult in fructose/streptozotocin diabetic rat model. Type 2 diabetes mellitus was induced by feeding sixty adult male albino rats with a high fructose diet accompanied by a single i.p. injection of streptozotocin (STZ). The rats were divided into 6 groups (10 rats/each) and the doses of nanoparticles were 10 mg/kg b.wt for ZnONPs, 1 mg/kg b.wt for CrO, and 0.4 mg/kg b.wt for SeNPs. The results displayed that diabetes significantly decreased bodyweight, serum insulin, C-peptide, adiponectin levels, erythrocyte glutathione peroxidase, serum superoxide dismutase activities, high-density lipoprotein cholesterol (HDL-C), and total antioxidant capacity while causing a substantial increase in serum glucose, C-reactive protein, atherogenic index, HOMA-IR, malondialdehyde, lipid profile, interleukin-6 levels, and liver function and kidney function parameters. Furthermore, the findings showed a decrease in insulin receptor substrate-1 (IRS-1) hepatic mRNA expression level and peroxisome proliferator-activated receptor (PPAR-γ) adipocyte mRNA expression level in type 2 diabetic rats. DNA damage was confirmed by performing the comet assay. Moreover, histological observation of pancreatic and hepatic tissues was performed, which were consistent with the biochemical results. The present study confirmed that oral administration of ZnONPs, CrONPs, SeNPs, and their mixture improved all the biochemical and genetic parameters toward normal levels and ameliorated the diabetic consequences that were manifested by restricting cellular DNA damage which maintaining pancreatic and hepatic tissues from oxidative damage. The best reported antidiabetic effect was observed in the mixture administered group.
本研究旨在评估单独或联合给予氧化锌纳米粒子（ZnONPs）、氧化铬纳米粒子（CrONPs）和硒纳米粒子（SeNPs）纳米粒子对果糖/链脲佐菌素糖尿病大鼠模型遗传和代谢损伤的抗糖尿病作用。2 型糖尿病通过用高果糖饮食喂养 60 只成年雄性白化大鼠并单次腹腔注射链脲佐菌素（STZ）诱导。将大鼠分为 6 组（每组 10 只），纳米粒子的剂量为 10mg/kg b.wt 的 ZnONPs、1mg/kg b.wt 的 CrO 和 0.4mg/kg b.wt 的 SeNPs。结果显示，糖尿病显著降低了体重、血清胰岛素、C 肽、脂联素水平、红细胞谷胱甘肽过氧化物酶、血清超氧化物歧化酶活性、高密度脂蛋白胆固醇（HDL-C）和总抗氧化能力，同时导致血清葡萄糖、C 反应蛋白、动脉粥样硬化指数、HOMA-IR、丙二醛、血脂谱、白细胞介素-6 水平以及肝功能和肾功能参数显著增加。此外，研究结果显示 2 型糖尿病大鼠肝胰岛素受体底物-1（IRS-1）mRNA 表达水平和脂肪细胞过氧化物酶体增殖物激活受体（PPAR-γ）mRNA 表达水平降低。通过彗星试验证实了 DNA 损伤。此外，还对胰腺和肝脏组织进行了组织学观察，这些结果与生化结果一致。本研究证实，口服 ZnONPs、CrONPs、SeNPs 及其混合物可使所有生化和遗传参数恢复正常水平，并改善糖尿病的后果，表现为限制细胞 DNA 损伤，使胰腺和肝脏组织免受氧化损伤。在给予混合物的组中观察到了最好的抗糖尿病效果。
