Saha Alik, Chakrovorty Arnob, Bhattacharjee Banani, Nandi Sisir, Samadder Asmita
Department of Life Science, National Institute of Technology Rourkela, Rourkela, Odisha, 769008, India.
Cytogenetics and Molecular Biology Lab., Department of Zoology, University of Kalyani, Kalyani, Nadia, West Bengal, 741235, India.
Comb Chem High Throughput Screen. 2025;28(8):1290-1300. doi: 10.2174/0113862073294446240426112801.
Genotoxins are chemical constituents that damage DNA or chromosomal architecture, leading to alterations in the genetic level. Streptozotocin, a monofunctional nitrosourea derivative, is often utilized to induce diabetes mellitus in laboratory animals because of its detrimental effects on pancreatic cells. The purpose of this work was to investigate possible protective efficacy against the genotoxic effects of Streptozotocin.
This study examines the potentiality of curcumin, a phytoproduct with anti-genotoxic and anti-diabetic qualities, against streptozotocin-induced chromosomal abnormalities and DNA damage. The study evaluates the possible protective efficacy of curcumin, exploring a variety of biochemical and molecular biology techniques in murine models.
The experiment involves a control group, the administration of Streptozotocin (Group S), and a curcumin-pretreated STZ group (Group SC) in mice. The effects of curcumin on DNA damage and chromosomal aberrations were investigated by histopathology, immunofluorescence, evaluation of chromosomal aberrations, detection of Reactive oxygen species, cell viability analysis, and DNA laddering assay.
The results demonstrated a significant reduction in DNA damage, chromosomal anomalies, and a decrease in the expression of the p53 protein in the curcumin-treated animals compared to that of STZ-treated mice.
The overall results show that curcumin can reduce chromosomal aberrations and DNA damage by altering the expression of p53 repair proteins. This suggests that curcumin has a promising future as a therapeutic agent, especially when it comes to drug-induced toxicity and the development of novel therapeutics.
基因毒素是能够破坏DNA或染色体结构，导致基因水平改变的化学成分。链脲佐菌素是一种单功能亚硝基脲衍生物，因其对胰腺细胞的有害作用，常用于在实验动物中诱导糖尿病。这项工作的目的是研究对链脲佐菌素基因毒性作用可能的保护效果。
本研究考察姜黄素这种具有抗基因毒性和抗糖尿病特性的植物产物，对链脲佐菌素诱导的染色体异常和DNA损伤的潜在作用。该研究评估姜黄素可能的保护效果，在小鼠模型中探索多种生物化学和分子生物学技术。
实验涉及小鼠中的一个对照组、链脲佐菌素给药组（S组）和姜黄素预处理的链脲佐菌素组（SC组）。通过组织病理学、免疫荧光、染色体畸变评估、活性氧检测、细胞活力分析和DNA梯状条带分析，研究姜黄素对DNA损伤和染色体畸变的影响。
结果表明，与链脲佐菌素处理的小鼠相比，姜黄素处理的动物中DNA损伤、染色体异常显著减少，p53蛋白表达降低。
总体结果表明，姜黄素可通过改变p53修复蛋白的表达来减少染色体畸变和DNA损伤。这表明姜黄素作为一种治疗剂具有广阔的前景，特别是在药物诱导的毒性和新型治疗方法的开发方面。
