Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Institute of Genetics and Regenerative Biology, College of Life Sciences, Zhejiang University, Hangzhou 310012, Zhejiang, China.
J Agric Food Chem. 2021 May 12;69(18):5306-5317. doi: 10.1021/acs.jafc.0c08147. Epub 2021 Apr 28.
Barley has abundant anthocyanin-rich accessions, which renders it an ideal model to investigate the regulatory mechanism of anthocyanin biosynthesis. This study functionally characterized two transcription factors: Ant1 and Ant2. Sequence alignment showed that the coding sequences of 1 and 2 are conserved among 11 colored hulless barley and noncolored barley varieties. The expression profiles of 1 and 2 were divergent between species, and significantly higher expression was found in two colored Qingke accessions. The co-expression of 1 and 2 resulted in purple pigmentation in transient transformation systems via the promotion of the transcription of four structural genes. Ant1 interacted with Ant2, and overexpression of 1 activated the transcription of 2. Moreover, overexpression of 1 led to anthocyanin accumulation in the pericarp and aleurone layer of transgenic barley grains. Overall, our results suggest that anthocyanin-enriched barley grains can be produced by manipulating 1 expression.
大麦富含富含花色苷的品种，这使其成为研究花色苷生物合成调控机制的理想模型。本研究对两个转录因子 Ant1 和 Ant2 进行了功能表征。序列比对表明，1 和 2 的编码序列在 11 个有色裸大麦和非彩色大麦品种中是保守的。1 和 2 的表达谱在物种间存在差异，并且在两个有色青稞品种中发现了明显更高的表达。1 和 2 的共表达通过促进四个结构基因的转录导致瞬时转化系统中的紫色着色。Ant1 与 Ant2 相互作用，1 的过表达激活了 2 的转录。此外，1 的过表达导致转基因大麦籽粒的果皮和糊粉层中积累花色苷。总的来说，我们的结果表明，通过操纵 1 的表达可以生产富含花色苷的大麦籽粒。
