McKeon C, Thiele C J, Ross R A, Kwan M, Triche T J, Miser J S, Israel M A
Division of Cancer Treatment, National Cancer Institute, Bethesda, Maryland 20892.
Cancer Res. 1988 Aug 1;48(15):4307-11.
Genetic characterization of human tumors promises new insights of biological importance and clinical relevance. We have found that two solid tumors, peripheral neuroepithelioma and Ewing's sarcoma of bone, which share a common cytogenetic rearrangement, are characterized by an indistinguishable and highly reproducible pattern of protooncogene expression. c-myc, N-myc, c-myb, and c-mil/raf-1 are all expressed at similar levels in these tumors. c-fes and c-sis expression was not detected in any specimens of either tumor. In contrast, the protooncogene c-ets-1, located near the breakpoint of the chromosomal translocation in these tumors, is variable in its expression. We also detected high levels of choline acetyltransferase in these tumors, which suggests a common neural origin. Since it is likely that the clinical behavior and therapeutic responsiveness of tumors relate closely to their biological and genetic features, the pattern of protooncogene expression of individual tumors may provide a novel basis for their characterization.
人类肿瘤的基因特征有望带来具有生物学重要性和临床相关性的新见解。我们发现，两种实体瘤，即外周神经上皮瘤和骨尤文肉瘤，它们具有共同的细胞遗传学重排，其特征是原癌基因表达模式难以区分且高度可重复。c-myc、N-myc、c-myb和c-mil/raf-1在这些肿瘤中的表达水平相似。在这两种肿瘤的任何标本中均未检测到c-fes和c-sis的表达。相比之下，位于这些肿瘤染色体易位断点附近的原癌基因c-ets-1，其表达是可变的。我们还在这些肿瘤中检测到高水平的胆碱乙酰转移酶，这表明它们有共同的神经起源。由于肿瘤的临床行为和治疗反应性可能与其生物学和基因特征密切相关，单个肿瘤的原癌基因表达模式可能为其特征描述提供新的依据。
