College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China.
Food Funct. 2021 May 21;12(10):4392-4401. doi: 10.1039/d1fo00424g. Epub 2021 Apr 28.
Selenomethionine (SeMet) has antioxidant and anti-inflammatory effects, as a widely used organic Se source in food supplements, and its inhibitory effect on the prevention and treatment of renal inflammatory injury is unclear. Here, in order to explore the protective effect of SeMet on kidney tissue of broilers and determine its potential molecular mechanism, we took broilers as the research object, lipopolysaccharide (LPS) was used as the source of stimulation, and the model was established by adding SeMet to the diet. The histopathological observation indicated that SeMet alleviated the LPS-induced characteristic changes of renal inflammatory injury. Besides, SeMet inhibited LPS-induced PI3K, AKT, caspase 8 and IκB-α downregulation, the necroptosis marker genes (FADD, RIP1, RIP3, MLKL and TNF-α), pro-inflammatory factors (NF-κB, PTGEs, COX-2, iNOS, IL-1β and IL-6) and HSP60, HSP70 and HSP90 overexpression. We concluded that SeMet ameliorates LPS-induced renal inflammatory injury in broilers by inhibiting necroptosis via the regulation of the PI3K/Akt pathway. Thus, we speculated that dietary SeMet may be a potential new strategy for the treatment of renal injury.
硒代蛋氨酸（SeMet）具有抗氧化和抗炎作用，作为一种广泛应用于食品补充剂的有机硒源，其对预防和治疗肾炎症损伤的抑制作用尚不清楚。在这里，为了探讨 SeMet 对肉鸡肾脏组织的保护作用，并确定其潜在的分子机制，我们以肉鸡为研究对象，以脂多糖（LPS）作为刺激源，通过在饮食中添加 SeMet 来建立模型。组织病理学观察表明，SeMet 减轻了 LPS 诱导的肾炎症损伤的特征性变化。此外，SeMet 抑制了 LPS 诱导的 PI3K、AKT、caspase 8 和 IκB-α 的下调，以及坏死凋亡标记基因（FADD、RIP1、RIP3、MLKL 和 TNF-α）、促炎因子（NF-κB、PTGEs、COX-2、iNOS、IL-1β 和 IL-6）和 HSP60、HSP70 和 HSP90 的过度表达。我们得出结论，SeMet 通过调节 PI3K/Akt 通路抑制坏死凋亡，从而改善 LPS 诱导的肉鸡肾炎症损伤。因此，我们推测膳食 SeMet 可能是治疗肾损伤的一种有潜力的新策略。
