Department of Research & Development, Restek Corporation, Bellefonte, Pennsylvania, USA.
J Sep Sci. 2021 Jul;44(13):2564-2576. doi: 10.1002/jssc.202001265. Epub 2021 May 24.
Cannabis legalization has led to the development of a variety of cannabis-infused products with edibles being one of the most popular. The state of California has implemented comprehensive cannabis testing regulations requiring the analysis of cannabinoids (potency) and contaminants, such as pesticides and mycotoxins, in any type of cannabis good. In this work, we propose an analytical workflow for the quantification of the California list of pesticides and mycotoxins, as well as six cannabinoids, in chocolate, using 3 mL of solvent for the extraction. For the analysis of pesticides and mycotoxins, clean-up steps employing a C18 solid-phase extraction cartridge and dispersive solid-phase extraction sorbents were implemented. Gas chromatography amenable pesticides were analyzed using low-pressure gas chromatography coupled to tandem mass spectrometry which allowed for a total method run of 12 min. Both liquid chromatography and gas chromatography instrumental methods had the same analysis time, ensuring satisfactory sample throughput. For the determination of cannabinoids, a dilution of the original organic extract collected for pesticides and mycotoxins analysis (and prior to any clean-up step) was used. Excellent results in terms of analytical figures of merit were obtained for all target analytes.
大麻合法化导致了各种含有大麻的产品的发展，其中可食用产品是最受欢迎的一种。加利福尼亚州已经实施了全面的大麻测试法规，要求对任何类型的大麻制品中的大麻素（效力）和污染物（如农药和霉菌毒素）进行分析。在这项工作中，我们提出了一种分析工作流程，用于定量分析巧克力中的加州农药和霉菌毒素列表，以及六种大麻素，使用 3 毫升溶剂进行提取。对于农药和霉菌毒素的分析，采用 C18 固相萃取小柱和分散固相萃取吸附剂进行净化步骤。可进行低压气相色谱分析的农药采用串联质谱法与低压气相色谱法相结合进行分析，总方法运行时间为 12 分钟。液体色谱和气相色谱仪器方法的分析时间相同，确保了令人满意的样品通量。对于大麻素的测定，使用了原始有机提取物的稀释液（用于农药和霉菌毒素分析，且在任何净化步骤之前）。所有目标分析物都获得了出色的分析质量指标结果。
