Maeda T, Matsuzaki H, Sekine T
Central Clinical Laboratories, Tokyo Metropolitan Police Hospital, Japan.
Clin Chem. 1988 Jul;34(7):1392-5.
We describe a simple, rapid, and less interference-susceptible method for using the Porter-Silber reaction for determination of urinary 17-hydroxycorticosteroids. The procedure is based on butanol extraction of the steroids after the urine sample is treated with potassium permanganate and sodium bisulfite. These treatments, together with an additional acid- and alkali-washing of the extract, could eliminate most of the substances in urine that interfere with the Porter-Silber reactions. Values so obtained correlated well (r = 0.95) with those by Furuya's method in which beta-glucuronidase is used. Our method may be useful and suitable for a screening test of adrenocortical and pituitary functions.
我们描述了一种简单、快速且不易受干扰的方法，用于利用波特-西尔伯反应测定尿中17-羟皮质类固醇。该方法基于在尿样用高锰酸钾和亚硫酸氢钠处理后，用丁醇萃取类固醇。这些处理，再加上对萃取物进行额外的酸洗和碱洗，可消除尿中大多数干扰波特-西尔伯反应的物质。如此获得的值与使用β-葡萄糖醛酸酶的古谷法所得值相关性良好（r = 0.95）。我们的方法可能有用且适用于肾上腺皮质和垂体功能的筛查试验。
