Ariyoshi Y, Osawa Y
Clin Chem. 1976 Feb;22(2):232-4.
We describe a simple, rapid, reliable method for determining urinary 17-hydroxycorticosteroids. A neutral resin (Amberlite XAD-2), which is a non-ionic cross-linked polystyrene, is used to extract and concentrate the steroids, which are then quantitatively determined with the Porter-Silber reaction. Use of the resin eliminates the need for enzymatic hydrolysis and n-butanol extractions, thereby decreasing analysis time considerably; results can be obtained within 3 h of receipt of specimens. Most of the nonsteroidal Porter-Silber chromogens are removed, resulting in a method that is highly specific and sensitive, even at low concentrations (0.6 mg/liter), and so more accurate and reproducible than currently used methods.
我们描述了一种测定尿17-羟皮质类固醇的简单、快速、可靠的方法。一种中性树脂（Amberlite XAD-2），它是一种非离子交联聚苯乙烯，用于提取和浓缩类固醇，然后用波特-西尔伯反应进行定量测定。使用该树脂无需进行酶水解和正丁醇萃取，从而大大缩短了分析时间；在收到标本后3小时内即可获得结果。大部分非甾体类波特-西尔伯色原被去除，从而得到一种高度特异且灵敏的方法，即使在低浓度（0.6毫克/升）下也是如此，因此比目前使用的方法更准确、更具可重复性。
