Johnson R N, Baker J R
Department of Clinical Biochemistry, Green Lane Hospital, Auckland, New Zealand.
Clin Chem. 1988 Jul;34(7):1456-9.
We compared thiobarbituric acid colorimetry and boronate chromatography for measuring glycation of serum proteins. With 14C-glycated human albumin as a test material, both methods were acceptably linear and precise. However, comparable estimates (mmol/L) of albumin glycation ranged from 0.22 for thiobarbituric acid to 0.05 for boronate, representing yields relative to [14C]glycosylprotein of 42% and 10%, respectively. The low yield with thiobarbituric acid was corroborated independently on the basis of kinetic differences between the reactions of fructose standards and of glycosylprotein, leading to underestimation of glycosylprotein concentration. The lower estimate of glycosylprotein by boronate chromatography was related to an apparent requirement for two [14C]glyco groups per albumin molecule to effect binding.
我们比较了硫代巴比妥酸比色法和硼酸酯色谱法测定血清蛋白糖化水平。以14C标记的糖化人白蛋白作为测试材料，两种方法都具有可接受的线性和精密度。然而，白蛋白糖化水平的可比估计值（mmol/L）范围为硫代巴比妥酸法的0.22至硼酸酯法的0.05，分别相当于相对于[14C]糖蛋白的产率为42%和10%。基于果糖标准品和糖蛋白反应之间的动力学差异，硫代巴比妥酸法的低产率得到了独立验证，这导致了糖蛋白浓度的低估。硼酸酯色谱法对糖蛋白的较低估计与每个白蛋白分子明显需要两个[14C]糖基以实现结合有关。
