Rendell M, Kao G, Mecherikunnel P, Petersen B, Duhaney R, Nierenberg J, Rasbold K, Klenk D, Smith P K
Clin Chem. 1985 Feb;31(2):229-34.
Two techniques originally developed for measurement of glycated ("glycosylated") hemoglobin but also applicable to determination of glycated albumin are the thiobarbituric acid colorimetric technique (I) and the aminophenylboronic acid affinity chromatographic procedure (II). The latter reliably distinguishes diabetics from nondiabetics, and concentrations of glycated hemoglobin and glycated albumin are linearly correlated. I is nonspecific; it neither correlates with diabetic status nor with values derived via the affinity technique. Most of the chromogenic material is present in the fraction of albumin that does not bind to aminophenylboronic acid. Glucose interferes significantly with I but only slightly with II. Prolonged incubation of plasma with glucose dramatically increases the II-determined glycated albumin. Reactivity with thiobarbituric acid increases much less, and mainly in the II-bound fraction. This fraction contains a high proportion of nonspecifically reactive material. The percentage of glycated albumin determined in crude plasma samples by II differs only slightly from the value determined by purifying the albumin from the plasma. This technique appears more promising than I for eventual clinical applications.
最初开发用于测量糖化（“糖基化”）血红蛋白但也适用于测定糖化白蛋白的两种技术是硫代巴比妥酸比色法（I）和氨基苯硼酸亲和色谱法（II）。后者能可靠地区分糖尿病患者和非糖尿病患者，且糖化血红蛋白和糖化白蛋白的浓度呈线性相关。方法I不具有特异性；它既与糖尿病状态无关，也与通过亲和技术获得的值无关。大多数发色物质存在于不与氨基苯硼酸结合的白蛋白部分中。葡萄糖对方法I有显著干扰，但对方法II的干扰很小。血浆与葡萄糖长时间孵育会显著增加方法II测定的糖化白蛋白。与硫代巴比妥酸的反应性增加较少，且主要在与方法II结合的部分中。该部分含有高比例的非特异性反应物质。通过方法II在粗血浆样品中测定的糖化白蛋白百分比与通过从血浆中纯化白蛋白测定的值仅略有不同。对于最终的临床应用，该技术似乎比方法I更有前景。
