Department of Biological Sciences, Royal Holloway University of London, Egham, UK.
Plant J. 2022 Jun;110(5):1516-1528. doi: 10.1111/tpj.15745. Epub 2022 Apr 7.
Sustainable production of chemicals and improving these biosources by engineering metabolic pathways to create efficient plant-based biofactories relies on the knowledge of available chemical/biosynthetic diversity present in the plant. Nicotiana species are well known for their amenability towards transformation and other new plant breeding techniques. The genus Nicotiana is primarily known through Nicotiana tabacum L., the source of tobacco leaves and all respective tobacco products. Due to the prevalence of the latter, N. tabacum and related Nicotiana species are one of the most extensively studied plants. The majority of studies focused solely on N. tabacum or other individual species for chemotyping. The present study analysed a diversity panel including 17 Nicotiana species and six accessions of Nicotiana benthamiana and created a data set that effectively represents the chemotype core collection of the genus Nicotiana. The utilisation of several analytical platforms and previously published libraries/databases enabled the identification and measurement of over 360 metabolites of a wide range of chemical classes as well as thousands of unknowns with dedicated spectral and chromatographic properties.
可持续地生产化学品，并通过工程代谢途径来改善这些生物资源，以创建高效的基于植物的生物工厂，这依赖于对植物中现有化学/生物合成多样性的了解。烟草属植物因其易于转化和其他新的植物育种技术而广为人知。烟草属主要以烟草（Nicotiana tabacum L.）为代表，它是烟叶和所有相关烟草产品的来源。由于后者的普遍存在，烟草（Nicotiana tabacum）和相关的烟草属植物是研究最多的植物之一。大多数研究仅专注于烟草（Nicotiana tabacum）或其他个别物种的化学型研究。本研究分析了一个包括 17 种烟草属植物和 6 个 Nicotiana benthamiana 品系的多样性面板，并创建了一个数据集，该数据集有效地代表了烟草属的化学型核心收集。利用多个分析平台和以前发表的文库/数据库，能够鉴定和测量 360 多种不同化学类别的代谢产物，以及具有专门光谱和色谱特性的数千种未知物。
