Department of Science and Technology, Federal University of São Paulo (Unifesp), São José dos Campos, Brazil.
Department of Medicine, Federal University of São Paulo (Unifesp), São Paulo, Brazil.
Sci Rep. 2021 Apr 28;11(1):9189. doi: 10.1038/s41598-021-88497-w.
Type 1 diabetes (T1DM) is a chronic disease characterized by hyperglycemia due to a deficiency in endogenous insulin production, resulting from pancreatic beta cell death. Persistent hyperglycemia leads to enhanced oxidative stress and liver injury. Several studies have evaluated the anti-diabetic and protective effects of probiotic strains in animal models. In the present study, we investigated, through histopathological and biochemical analyses, the effects of eight weeks of administration of Saccharomyces boulardii (S. boulardii) yeast on the liver of streptozotocin (STZ) induced diabetic C57BL/6 mice. Our results demonstrated that S. boulardii attenuates hepatocytes hydropic degeneration and hepatic vessels congestion in STZ-induced diabetic mice. The treatment attenuated the oxidative stress in diabetic mice leading to a reduction of carbonylated protein concentration and increased activity of antioxidant enzymes superoxide dismutase and glutathione peroxidase, compared to untreated diabetic animals. The results also show the beneficial influence of S. boulardii in regulating the hepatic concentration of renin angiotensin system (RAS) peptides. Therefore, our results demonstrated that S. boulardii administration to STZ-induced diabetic mice reduces oxidative stress and normalizes the concentration of RAS peptides, supporting the hypothesis that this yeast may have a role as a potential adjunctive therapy to attenuate diabetes-induced liver injury.
1 型糖尿病（T1DM）是一种慢性疾病，其特征是由于内源性胰岛素产生不足导致的高血糖，这是由于胰腺β细胞死亡引起的。持续的高血糖会导致氧化应激增强和肝损伤。已有多项研究评估了益生菌菌株在动物模型中的抗糖尿病和保护作用。在本研究中，我们通过组织病理学和生化分析，研究了八周给予酿酒酵母（Saccharomyces boulardii，S. boulardii）对链脲佐菌素（streptozotocin，STZ）诱导的糖尿病 C57BL/6 小鼠肝脏的影响。我们的结果表明，S. boulardii 减轻了 STZ 诱导的糖尿病小鼠肝细胞水样变性和肝血管充血。与未治疗的糖尿病动物相比，该治疗减轻了糖尿病小鼠的氧化应激，导致羰基化蛋白浓度降低和抗氧化酶超氧化物歧化酶和谷胱甘肽过氧化物酶的活性增加。结果还表明 S. boulardii 对调节肝 renin-angiotensin 系统（RAS）肽浓度具有有益影响。因此，我们的结果表明，S. boulardii 给药于 STZ 诱导的糖尿病小鼠可减轻氧化应激并使 RAS 肽浓度正常化，这支持了该酵母可能作为减轻糖尿病引起的肝损伤的辅助治疗的假说。
