Huang Bo, Kang Bueom-Goo, Lim Soon Sung, Zhang Xian Hua
Department of Food Science and Engineering, Jinzhou Medical University, Jinzhou, China.
Department of Food Science and Nutrition, Hallym University, Chuncheon, Korea.
Ann Dermatol. 2019 Oct;31(5):502-510. doi: 10.5021/ad.2019.31.5.502. Epub 2019 Aug 30.
In our preliminary study, we screened for their potential to inhibit 5α-reductase, and (MF) extract showed the most potent activity as confirmed by high-performance liquid chromatography (HPLC).
This study aimed to investigate the effects of MF extract on 5α-reductase activity and its mechanisms of action in the prevention or treatment of androgenetic alopecia.
HPLC was used to measure 5α-reductase activity. The hair growth-promoting effect of MF extract in the shaved dorsal skin of C57BL/6J mice was studied for 30 days. Hair follicles were examined by histological examination. Protein and mRNA levels of growth factors involved in hair growth were determined by western blotting, and reverse transcription-polymerase chain reaction (RT-PCR) and qPCR, respectively. Cell proliferation was measured by (3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay.
MF extract at 0.5 mg/ml showed 43.5% inhibition of 5α-reductase. MF extract promoted hair growth by inducing anagen phase reflected by skin color, hair density, and the number and size of hair follicles. It not only reduces the expression of transforming growth factor-beta 1 (TGF-β1) and Dickkopf-1 (DKK-1), but also markedly upregulated insulin-like growth factor 1 and keratinocyte growth factor in the dorsal dermal tissue. Ursolic acid, ecdcysteron, and ergosterol peroxide were identified as active constituents by activity-guided fractionation to inhibit 5α-reductase. They decreased the gene expression of TGF-β1 and DKK-1 in human hair dermal papilla cells.
In summary, these finding indicate that MF extract might be a good drug candidate for hair growth promotion.
在我们的初步研究中，我们筛选了它们抑制5α-还原酶的潜力，高效液相色谱法（HPLC）证实，（MF）提取物显示出最有效的活性。
本研究旨在探讨MF提取物对5α-还原酶活性的影响及其在预防或治疗雄激素性脱发中的作用机制。
采用HPLC测定5α-还原酶活性。研究了MF提取物对C57BL/6J小鼠背部剃毛皮肤的促毛发生长作用，为期30天。通过组织学检查毛囊。分别采用蛋白质印迹法、逆转录-聚合酶链反应（RT-PCR）和定量PCR测定参与毛发生长的生长因子的蛋白质和mRNA水平。采用（3-（4,5-二甲基噻唑-2-基）-5-（3-羧甲氧基苯基）-2-（4-磺基苯基）-2H-四唑）法测定细胞增殖。
0.5mg/ml的MF提取物对5α-还原酶的抑制率为43.5%。MF提取物通过诱导生长期促进毛发生长，这通过皮肤颜色、毛发密度以及毛囊的数量和大小得以体现。它不仅降低了转化生长因子-β1（TGF-β1）和Dickkopf-1（DKK-1）的表达，还显著上调了背部真皮组织中胰岛素样生长因子1和角质形成细胞生长因子的表达。通过活性导向分级分离鉴定出熊果酸、蜕皮甾酮和过氧化麦角甾醇为抑制5α-还原酶的活性成分。它们降低了人毛发真皮乳头细胞中TGF-β1和DKK-1的基因表达。
总之，这些发现表明MF提取物可能是一种促进毛发生长的良好候选药物。
