Tian Peng, Xiao Jiaguang, Jia Zhiyu, Guo Feng, Wang Xiaolei, Wang Wei, Wang Jianjia, Huang Dingyong, Niu Wentao
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China Third Institute of Oceanography, Ministry of Natural Resources Xiamen China.
Biodivers Data J. 2021 Apr 19;9:e62395. doi: 10.3897/BDJ.9.e62395. eCollection 2021.
Complete mitochondrial DNA sequence data have played a significant role in phylogenetic and evolutionary studies of scleractinian corals. In this study, the complete mitogenome of Gardiner, 1898, collected from Guangdong Province, China, was sequenced by next-generation sequencing for the first time. is the first species for which a mitogenome has been sequenced in the family Psammocoridae. The length of its assembled mitogenome sequence was 16,274 bp, including 13 protein-coding genes, two tRNAs and two rRNAs. Its gene content and gene order were consistent with the other Scleractinia species. All genes were encoded on the H strand and the GC content of the mitochondrial genome was 30.49%. Gene content and order were consistent with the other Scleractinia species. Based on 13 protein-coding genes, Maximum Likelihood phylogenetic analysis showed that belongs to the "Robust" clade. Mitochondrial genome data provide important molecular information for understanding the phylogeny of stony corals. More variable markers and additional species should be sequenced to confirm the evolutionary relationships of Scleractinia in the future.
完整的线粒体DNA序列数据在石珊瑚的系统发育和进化研究中发挥了重要作用。在本研究中,首次通过下一代测序技术对采集自中国广东省的1898年加德纳石珊瑚(Gardiner, 1898)的完整线粒体基因组进行了测序。它是沙珊瑚科(Psammocoridae)中首个线粒体基因组被测序的物种。其组装后的线粒体基因组序列长度为16274碱基对,包括13个蛋白质编码基因、两个tRNA和两个rRNA。其基因组成和基因顺序与其他石珊瑚物种一致。所有基因都在重链上编码,线粒体基因组的GC含量为30.49%。基因组成和顺序与其他石珊瑚物种一致。基于13个蛋白质编码基因的最大似然系统发育分析表明,该物种属于“强壮”分支。线粒体基因组数据为理解石珊瑚的系统发育提供了重要的分子信息。未来应测序更多可变标记和更多物种,以确认石珊瑚的进化关系。