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(石珊瑚目,叶状珊瑚科)的完整线粒体基因组:线粒体基因组特征与系统发育定位

Complete mitochondrial genome of (Scleractinia, Lobophylliidae): Mitogenome characterization and phylogenetic positioning.

作者信息

Niu Wentao, Yu Shuangen, Tian Peng, Xiao Jiaguang

机构信息

Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, State Oceanic Administration Xiamen China.

出版信息

Zookeys. 2018 Oct 29(793):1-14. doi: 10.3897/zookeys.793.28977. eCollection 2018.

Abstract

Lack of mitochondrial genome data of Scleractinia is hampering progress across genetic, systematic, phylogenetic, and evolutionary studies concerning this taxon. Therefore, in this study, the complete mitogenome sequence of the stony coral (Ellis & Solander, 1786), has been decoded for the first time by next generation sequencing and genome assembly. The assembled mitogenome is 17,697 bp in length, containing 13 protein coding genes (PCGs), two transfer RNAs and two ribosomal RNAs. It has the same gene content and gene arrangement as in other Scleractinia. All genes are encoded on the same strand. Most of the PCGs use ATG as the start codon except for ND2, which uses ATT as the start codon. The A+T content of the mitochondrial genome is 65.92% (25.35% A, 40.57% T, 20.65% G, and 13.43% for C). Bayesian and maximum likelihood phylogenetic analysis have been performed using PCGs, and the result shows that clustered closely with (Sheppard & Salm, 1988), both of which belong to Lobophylliidae, when compared with species belonging to Merulinidae and other scleractinian taxa used as outgroups. The complete mitogenome of provides essential and important DNA molecular data for further phylogenetic and evolutionary analyses of corals.

摘要

石珊瑚线粒体基因组数据的缺失阻碍了有关该分类群的遗传、系统、系统发育和进化研究的进展。因此,在本研究中,首次通过下一代测序和基因组组装对石珊瑚(埃利斯和索兰德,1786年)的完整线粒体基因组序列进行了解码。组装后的线粒体基因组长度为17,697 bp,包含13个蛋白质编码基因(PCG)、两个转运RNA和两个核糖体RNA。它具有与其他石珊瑚相同的基因内容和基因排列。所有基因都编码在同一条链上。除了使用ATT作为起始密码子的ND2外,大多数PCG使用ATG作为起始密码子。线粒体基因组的A+T含量为65.92%(A为25.35%,T为40.57%,G为20.65%,C为13.43%)。使用PCG进行了贝叶斯和最大似然系统发育分析,结果表明,与用作外群的梅氏珊瑚科和其他石珊瑚分类群的物种相比, 与 (谢泼德和萨尔姆, 1988年)紧密聚类,二者均属于叶状珊瑚科。 的完整线粒体基因组为珊瑚的进一步系统发育和进化分析提供了重要的DNA分子数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/90da/6218560/41f88c4e3adb/zookeys-793-001-g001.jpg

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