Gupta Archana A, Kheur Supriya, Badhe Ravindra V, Raj A Thirumal, Bhonde Ramesh, Jaisinghani Amit, Vyas Nishant, Patil Vikrant R, Alhazmi Yaser Ali, Parveen Sameena, Baeshen Hosam Ali, Patil Shankargouda
Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, India.
Department of Pharmaceutical Sciences and Research, Dr. D.Y. Patil College of Pharmacy, Dr. D. Y. Patil Vidyapeeth, Pune.
Saudi J Biol Sci. 2021 Apr;28(4):2210-2215. doi: 10.1016/j.sjbs.2021.01.008. Epub 2021 Jan 20.
Vitamin D is a commonly used bone modulator in regenerative medicine. Several modalities have been explored for the delivery of vitamin D including nanoparticles and scaffold. The present study aimed to assess the potential use of a bio-degradable chitosan scaffold for the delivery of vitamin D. The objectives included fabrication of a bio-degradable chitosan scaffold, integration of vitamin D into the scaffold, characterization of the vitamin D integrated scaffold. Characterization was carried out using, X-ray diffraction, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The structure of the scaffold was assessed by scanning electron microscopy. The scaffold was placed in phosphate buffer saline and the release duration of vitamin D was observed using UV spectrophotometry. Dental pulp mesenchymal stem cells were added to the scaffold to study the scaffold associated toxicity and the functionality of the scaffold released vitamin D. The vitamin D release period from the scaffold was estimated to be for 80 hrs. MTT assay of the stem cells was comparable to that of the control group (stem cells cultured in media) inferring that the scaffold is not toxic towards the stem cells. The positive alizarin red S staining, a higher expression of alkaline phosphatase, osteocalcin, and RunX2 confirmed the functional capability (osteogenic differentiation of the stem cells) of the released vitamin D. Based on the data from the present study, it can be inferred that chitosan scaffold can be used for the sustained delivery of functional vitamin D for 3-5 days.
维生素D是再生医学中常用的骨调节剂。人们已经探索了多种维生素D递送方式，包括纳米颗粒和支架。本研究旨在评估可生物降解的壳聚糖支架用于递送维生素D的潜在用途。目标包括制备可生物降解的壳聚糖支架、将维生素D整合到支架中以及对整合了维生素D的支架进行表征。使用X射线衍射、傅里叶变换红外光谱和差示扫描量热法进行表征。通过扫描电子显微镜评估支架的结构。将支架置于磷酸盐缓冲盐水中，使用紫外分光光度法观察维生素D的释放持续时间。将牙髓间充质干细胞添加到支架中，以研究支架相关毒性以及支架释放的维生素D的功能。支架中维生素D的释放期估计为80小时。干细胞的MTT分析结果与对照组（在培养基中培养的干细胞）相当，这表明该支架对干细胞无毒。茜素红S染色阳性、碱性磷酸酶、骨钙素和RunX2的表达较高，证实了释放的维生素D的功能能力（干细胞的成骨分化）。根据本研究的数据，可以推断壳聚糖支架可用于功能性维生素D的持续递送3至5天。
