Seciu-Grama Ana-Maria, Lazăr Sorana Elena, Petrescu Simona, Mocioiu Oana Cătălina, Crăciunescu Oana, Atkinson Irina
National Institute of Research and Development for Biological Sciences, 296, Spl. Independentei, 060031 Bucharest, Romania.
"Ilie Murgulescu" Institute of the Physical Chemistry of the Romanian Academy, 202, Spl. Independentei, 060021 Bucharest, Romania.
J Funct Biomater. 2025 Apr 14;16(4):141. doi: 10.3390/jfb16040141.
Reconstruction of extensive bone defects due to age, trauma, or post-illness conditions remains challenging. Biomimetic scaffolds with osteogenic capabilities have been proposed as an alternative to the classical autograft and allograft implants. Three-dimensional scaffolds were obtained based on Ce-doped mesoporous bioactive glass (MBG) and (SA) as sacrificial templates functionalized with vitamin D. The study aimed to investigate the effect of vitamin D functionalization on the optimal variant of a 3D scaffold doped with 3 mol% ceria, selected in our previous work based on its biological and physicochemical properties. Scanning electron microscopy (SEM) images of the non-functionalized/functionalized scaffolds revealed a porous structure with interconnected pores ranging from 100 to 350 μm. Fourier transform infrared spectroscopy (FTIR) and SEM analysis confirmed the surface functionalization. Cytotoxicity evaluation showed that all investigated scaffolds do not exhibit cytotoxicity and genotoxicity toward the Saos-2 osteosarcoma cell line. Moreover, the study demonstrated that functionalization with vitamin D enhanced osteogenic activity in dental pulp stem cells (DPSCs) by increasing calcium deposition and osteocalcin secretion, as determined by Alizarin red stain and a colorimetric ELISA kit, as a result of its synergistic action with cerium ions. The results showed that the Ce-doped MBG scaffold functionalized with vitamin D had the potential for applications in bone regeneration.
由于年龄、创伤或疾病后状况导致的大面积骨缺损修复仍然具有挑战性。具有成骨能力的仿生支架已被提议作为传统自体移植和同种异体移植植入物的替代方案。基于铈掺杂介孔生物活性玻璃（MBG）和作为牺牲模板并用维生素D功能化的海藻酸钠（SA）制备了三维支架。本研究旨在探讨维生素D功能化对掺杂3 mol%二氧化铈的三维支架最佳变体的影响，该变体是我们在之前的工作中根据其生物学和物理化学性质选择的。未功能化/功能化支架的扫描电子显微镜（SEM）图像显示出具有相互连通的孔隙，孔径范围为100至350μm的多孔结构。傅里叶变换红外光谱（FTIR）和SEM分析证实了表面功能化。细胞毒性评估表明，所有研究的支架对Saos-2骨肉瘤细胞系均未表现出细胞毒性和遗传毒性。此外，该研究表明，由于维生素D与铈离子的协同作用，通过茜素红染色和比色ELISA试剂盒测定，维生素D功能化通过增加钙沉积和骨钙素分泌增强了牙髓干细胞（DPSC）中的成骨活性。结果表明，用维生素D功能化的铈掺杂MBG支架具有骨再生应用潜力。
