Association of polymorphisms in genes involved in enamel formation, taste preference and immune response with early childhood caries in Saudi pre-school children.

Dental caries is primarily elicited by modifiable factors such as inadequate oral hygiene, poor dietary practices and deficient fluoride exposure. However, there is a growing body of evidence suggesting the profound influence of genetic factors in dental caries susceptibility. The present study aimed to evaluate the association between single nucleotide polymorphisms (SNPs) in (rs12640848), (rs1784418), (rs713598), and (rs4547741) genes and early childhood caries (ECC) in Saudi preschool children This case-control study enrolled 360 Saudi preschool children (262 with ECC and 98 caries-free). Data on environmental factors were collected through a questionnaire. However, caries experience and oral hygiene data were obtained during clinical examination. Buccal swab samples were collected for DNA extraction and SNPs were genotyped using PCR and DNA sequencing. Children with ECC were compared to caries free children (control), then they were categorized into two categories based on ECC severity as follows; non-severe ECC (NS-ECC), and severe-ECC (S-ECC). Association between the SNPs, ECC, NS-ECC, and S-ECC was reported as an odds ratio (OR) with a 95% confidence interval (CI). The majority of the children (72.8%) exhibited ECC (31.7% NS-ECC and 41.1% S-ECC) with mean dmft of 4.20 ± 4.05. Multivariate analyses of environmental factors showed that nocturnal feeding was a risk factor for ECC ( = 0.008). Poor oral hygiene was also a risk factor for both NS-ECC and S-ECC (ECC:   0.0001, NS-ECC:  = 0.032 and S-ECC:  < 0.0001). Univariate analysis showed that the AG genotype of rs1784418 of gene was protective against ECC (OR = 0.532; 95% CI = 0.316-0.897,  = 0.018) and against NS-ECC (OR = 0.436; 95% CI = 0.238-0.798,  = 0.007). When environmental risk factors for ECC were included as covariates during multivariate analysis, AG variant in rs1784418 of gene remained less frequent in NS-ECC cases compared to controls with borderline significance (OR = 0.542; 95% CI = 0.285-1.033,  = 0.063). Our findings concluded that rs1784418 SNP might be associated with protection against ECC in Saudi preschool children.