Guangxi Medical University College of Stomatology, No.10 Shuangyong Road, Nanning, 530021, China.
Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China.
Clin Oral Investig. 2024 Aug 30;28(9):508. doi: 10.1007/s00784-024-05894-x.
The aim was to assess the associations between the LTF, MMP20, CA6, and TAS1R2 polymorphisms and caries in the Zhuang population and explore the underlying mechanism of the impact of lactoferrin on caries susceptibility.
A case-control study of 315 adolescents was conducted in Guangxi, China, from May-November 2022. Data were collected through oral examinations and questionnaires. Buccal mucosa cells and DNA samples were collected using the SNPscan technique. Saliva and supragingival plaque samples were taken from 69 subjects with various LTF rs10865941 genotypes. The relationships among the LTF rs10865941 polymorphism, lactoferrin, Streptococcus mutans, and caries were investigated by using the ELISA and qRT-PCR, along with logistic regression analysis.
The genotype distribution of the LTF gene were significantly different between the case and control groups (p = 0.018). The case group had lower C allele and greater T allele frequencies than the control group (p = 0.006). The LTF rs10865941 polymorphism was associated with caries in the codominant, dominant, and additive models (p < 0.05). MMP20 rs1784418, CA6 rs2274328, and TAS1R2 rs35874116 were not significantly different between the two groups (p > 0.05). A greater quantity of S. mutans. in the supragingival plaque was found in the case group (p = 0.03). There were significant differences between the two groups in both the codominant model and the dominant model (p < 0.05).
The LTF rs10865941 polymorphism may be associated with caries susceptibility in the Zhuang population of China. The LTF rs10865941 T allele may be a potential risk factor for dental caries.
评估 LTF、MMP20、CA6 和 TAS1R2 多态性与壮族人群龋齿的关系，并探讨乳铁蛋白影响龋齿易感性的潜在机制。
2022 年 5 月至 11 月，在中国广西进行了一项 315 名青少年的病例对照研究。通过口腔检查和问卷调查收集数据。采用 SNPscan 技术采集颊黏膜细胞和 DNA 样本。从 69 名具有不同 LTF rs10865941 基因型的受试者中采集唾液和龈上菌斑样本。采用 ELISA 和 qRT-PCR 以及逻辑回归分析，研究 LTF rs10865941 多态性、乳铁蛋白、变形链球菌与龋齿之间的关系。
LTF 基因的基因型分布在病例组和对照组之间存在显著差异（p=0.018）。病例组的 C 等位基因和 T 等位基因频率均低于对照组（p=0.006）。LTF rs10865941 多态性与病例组和对照组的显性、共显性和加性模型均相关（p<0.05）。MMP20 rs1784418、CA6 rs2274328 和 TAS1R2 rs35874116 两组间无显著性差异（p>0.05）。病例组龈上菌斑中变形链球菌数量较多（p=0.03）。两组在共显性模型和显性模型中均有显著差异（p<0.05）。
LTF rs10865941 多态性可能与中国壮族人群的龋齿易感性有关。LTF rs10865941 T 等位基因可能是龋齿的潜在危险因素。
