Department of Paediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Department of Stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Oral Dis. 2018 Nov;24(8):1545-1553. doi: 10.1111/odi.12939. Epub 2018 Aug 20.
Dental caries is related to cariogenic bacteria, salivary components, oral hygiene and host susceptibility. Lactoferrin is an important antimicrobial glycoprotein in saliva; however, the role of the LTF gene in caries susceptibility is unclear. We investigated the association between LTF polymorphisms and the severity of caries.
Our study included 910 healthy paediatric subjects (aged 24-48 months) categorised into three groups: 403 with no caries or white-spot lesions; 230 with moderate caries (8 ≤ dmft ≤ 12); and 277 with severe caries (13 ≤ dmft ≤ 20). Information regarding the subjects' oral habits was gathered using questionnaires. The LTF rs1126477 and rs1126478 polymorphism alleles were genotyped by Sanger sequencing.
The three groups showed no significant differences in LTF polymorphisms alleles, genotypes or haplotypes distribution. Multifactor dimensionality reduction analysis showed that the interactions between breastfeeding for a duration >24 months, night feeding >24 months and high frequency of sweet food intake increased the risk of caries (p = 0.0014); however, we detected no interaction effect between the LTF polymorphisms and oral habits on caries susceptibility.
The LTF rs1126477 and rs1126478 polymorphisms showed no association with the different levels of caries risk in our Chinese paediatric cohort.
龋齿与致龋菌、唾液成分、口腔卫生和宿主易感性有关。乳铁蛋白是唾液中一种重要的抗菌糖蛋白；然而，LTF 基因在龋齿易感性中的作用尚不清楚。我们研究了 LTF 多态性与龋齿严重程度之间的关系。
本研究纳入了 910 名健康的儿科受试者（年龄 24-48 个月），分为三组：403 名无龋齿或白垩斑；230 名患有中度龋齿（8≤dmft≤12）；277 名患有严重龋齿（13≤dmft≤20）。使用问卷收集了受试者口腔习惯的信息。通过 Sanger 测序对 LTF rs1126477 和 rs1126478 多态性等位基因进行基因分型。
三组之间 LTF 多态性等位基因、基因型或单倍型分布无显著差异。多因子降维分析显示，母乳喂养时间>24 个月、夜间喂养时间>24 个月和甜食摄入频率高之间的相互作用增加了龋齿的风险（p=0.0014）；然而，我们未检测到 LTF 多态性与口腔习惯对龋齿易感性的相互作用效应。
在中国儿科队列中，LTF rs1126477 和 rs1126478 多态性与不同水平的龋齿风险无关。
