Postgraduate Program in Hepatobiliary/Pancreatic Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
2nd Department of Surgery and Laboratory of Experimental Surgery, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Pan Afr Med J. 2021 Feb 5;38:132. doi: 10.11604/pamj.2021.38.132.20202. eCollection 2021.
we present a rat experimental model used to evaluate the possible reduction in the extent of pancreatic tissue injury in acute pancreatitis cases, after administration of eugenol.
one hundred and twenty Wistar rats were used, which were randomly assigned in 3 groups: sham (n=20), control (n=50) and eugenol (n=50). Acute pancreatitis was induced by biliopancreatic ligation in the control and eugenol groups, but not in the Sham group. In the eugenol group, eugenol was administered per-os. Five histopathological parameters, such as edema, inflammatory infiltration, duct dilatation, hemorrhage and acinar necrosis were evaluated.
at 72 h from acute pancreatitis induction, the total histological score was diminished in the eugenol group (p<0.0005) and duct dilatation and inflammatory infiltration were reduced compared to the control group (p<0.05). In addition, at 72 h, eugenol reduced pancreatic myeloperoxidase activity (p<0.0005).
eugenol, a highly free radical scavenger agent, may have a preventive role in acute pancreatic injury, as it was evident in our rat experimental model.
我们提出了一种大鼠实验模型，用于评估在急性胰腺炎病例中，给予丁香酚后，胰腺组织损伤程度可能会降低。
使用了 120 只 Wistar 大鼠，将它们随机分为 3 组：假手术组（n=20）、对照组（n=50）和丁香酚组（n=50）。在对照组和丁香酚组中通过胆胰管结扎诱导急性胰腺炎，但在假手术组中不进行。在丁香酚组中，通过口服给予丁香酚。评估了 5 种组织病理学参数，如水肿、炎症浸润、导管扩张、出血和腺泡坏死。
在急性胰腺炎诱导后 72 小时，丁香酚组的总组织学评分降低（p<0.0005），与对照组相比，导管扩张和炎症浸润减少（p<0.05）。此外，在 72 小时时，丁香酚降低了胰腺髓过氧化物酶活性（p<0.0005）。
丁香酚作为一种高度的自由基清除剂，可能在急性胰腺损伤中具有预防作用，这在我们的大鼠实验模型中得到了证实。
