蛋白酶体抑制剂可改善大鼠重症急性胰腺炎及相关肺损伤。
Proteasome inhibitor ameliorates severe acute pancreatitis and associated lung injury of rats.
作者信息
Chen Xi, Li Shun-Le, Wu Tao, Liu Ji-Dong
机构信息
Department of General Surgery, The Second Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2008 May 28;14(20):3249-53. doi: 10.3748/wjg.14.3249.
AIM
To observe the effect of proteasome inhibitor MG-132 on severe acute pancreatitis (SAP) and associated lung injury of rats.
METHODS
Male adult SD rats were randomly divided into SAP group, sham-operation group, and MG-132 treatment group. A model of SAP was established by injection of 5% sodium taurocholate into the biliary-pancreatic duct of rats. The MG-132 group was pretreated with 10 mg/kg MG-132 intraperitoneally (ip) 30 min before the induction of pancreatitis. The changes in serum amylase, myeloperoxidase (MPO) activity of pancreatic and pulmonary tissue were measured. The TNF-alpha level in pancreatic cytosolic fractions was assayed with an enzyme-linked immunosorbent assay (ELISA) kit. Meanwhile, the pathological changes in both pancreatic and pulmonary tissues were also observed.
RESULTS
MG-132 significantly decreased serum amylase, pancreatic weight/body ratio, pancreatic TNF-alpha level, pancreatic and pulmonary MPO activity (P < 0.05). Histopathological examinations revealed that pancreatic and pulmonary samples from rats pretreated with MG-132 demonstrated milder edema, cellular damage, and inflammatory activity (P < 0.05).
CONCLUSION
The proteasome inhibitor MG-132 shows a protective effect on severe acute pancreatitis and associated lung injury of rats.
目的
观察蛋白酶体抑制剂MG - 132对大鼠重症急性胰腺炎（SAP）及相关肺损伤的影响。
方法
将成年雄性SD大鼠随机分为SAP组、假手术组和MG - 132治疗组。通过向大鼠胆胰管注射5%牛磺胆酸钠建立SAP模型。在诱导胰腺炎前30分钟，MG - 132组腹腔注射10mg/kg MG - 132进行预处理。检测血清淀粉酶、胰腺和肺组织髓过氧化物酶（MPO）活性的变化。用酶联免疫吸附测定（ELISA）试剂盒检测胰腺细胞溶质部分的肿瘤坏死因子-α（TNF-α）水平。同时，观察胰腺和肺组织的病理变化。
结果
MG - 132显著降低血清淀粉酶、胰腺重量/体重比、胰腺TNF-α水平、胰腺和肺MPO活性（P < 0.05）。组织病理学检查显示，MG - 132预处理大鼠的胰腺和肺样本水肿、细胞损伤及炎症活性较轻（P < 0.05）。
结论
蛋白酶体抑制剂MG - 132对大鼠重症急性胰腺炎及相关肺损伤具有保护作用。
Zotero 插件