Acri Timothy M, Laird Noah Z, Hong Liu, Chakka Jaidev L, Shin Kyungsup, Elangovan Satheesh, Salem Aliasger K
University of Iowa, College of Pharmacy, 115 S. Grand Avenue Iowa City, Iowa.
University of Iowa, College of Dentistry, 801 Newton Road Iowa City, Iowa.
MRS Adv. 2019 Dec;4(64):3505-3512. doi: 10.1557/adv.2020.61. Epub 2019 Sep 9.
This work focuses on the development of a system to control the formation of bone to complement developments that have enabled potent regeneration of bony tissue. Scaffolds were fabricated with chemically modified RNA encoding for bone morphogenetic protein-9 (cmBMP9) and capped with salicylic acid (SA)-containing polymer (SAPAE). The goal was to determine if SAPAE could inhibit the formation of bone in a pilot animal study since cmBMP9 has been demonstrated to be highly effective in regenerating bone in a rat calvarial defect model. The results indicated that cmBMP9 increased bone formation (30% increase in area covered compared to control) and that SAPAE trended toward reducing the bone formation. These results suggest SAPAE could be useful as a chemical agent in reducing unwanted bone formation in implants loaded with cmBMP9.
这项工作聚焦于开发一个控制系统，以促进骨形成，从而补充能够实现骨组织高效再生的相关进展。研究人员制备了含有编码骨形态发生蛋白-9（cmBMP9）的化学修饰RNA的支架，并在其表面覆盖了含水杨酸（SA）的聚合物（SAPAE）。由于cmBMP9已被证明在大鼠颅骨缺损模型中对骨再生非常有效，因此本研究的目的是在一项初步动物实验中确定SAPAE是否能够抑制骨形成。结果表明，cmBMP9促进了骨形成（与对照组相比，覆盖面积增加了30%），而SAPAE则有减少骨形成的趋势。这些结果表明，SAPAE作为一种化学试剂，可能有助于减少植入cmBMP9时不必要的骨形成。
