使用荧光报告基因端粒酶永生化人成纤维细胞系检测单个细胞干扰素-β的产生。
Detecting single cell interferon-beta production using a fluorescent reporter telomerase-immortalized human fibroblast cell line.
机构信息
Health Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
出版信息
STAR Protoc. 2021 Apr 10;2(2):100436. doi: 10.1016/j.xpro.2021.100436. eCollection 2021 Jun 18.
Recent data suggest that cells respond to infection by upregulating the antiviral cytokine interferon-beta (IFN-ß) in a fraction of infected cells. Approaches are thus needed to study these responses on a single-cell level rather than bulk population. Here, we describe a protocol to analyze the IFN-ß response of individual cells using flow cytometry and immunofluorescence microscopy. We show the heterogeneous IFN-ß response to inactivated Sendai virus and human cytomegalovirus, but this protocol can be adapted to other viruses. For complete details on the use and execution of this protocol, please refer to Hare et al. (2020).
最近的数据表明，细胞通过在部分感染细胞中上调抗病毒细胞因子干扰素-β（IFN-ß）来对感染做出反应。因此，需要采用一种方法在单细胞水平上而不是在细胞群体水平上研究这些反应。在这里，我们描述了一种使用流式细胞术和免疫荧光显微镜分析单个细胞中 IFN-ß 反应的方案。我们展示了对灭活的仙台病毒和人巨细胞病毒的异质 IFN-ß 反应，但此方案可以适应其他病毒。有关此方案的使用和执行的完整详细信息，请参见 Hare 等人。（2020 年）。
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