野生型和突变型人类巨细胞病毒在寿命延长的人二倍体成纤维细胞中的复制
Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts.
作者信息
Bresnahan W A, Hultman G E, Shenk T
机构信息
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA.
出版信息
J Virol. 2000 Nov;74(22):10816-8. doi: 10.1128/jvi.74.22.10816-10818.2000.
Abstract
A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.
摘要
编码人端粒酶催化亚基的cDNA被用于产生原代人二倍体成纤维细胞的寿命延长衍生物。这些寿命延长的细胞支持人巨细胞病毒（HCMV）的高效复制。产生了一个包含HCMV UL82基因的寿命延长细胞的亚克隆，并用于分离和繁殖一种在低感染复数感染后表现出严重生长缺陷的病毒。
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