College of Food Science, Sichuan Agricultural University, Ya'an, China.
J Food Biochem. 2021 Jun;45(6):e13746. doi: 10.1111/jfbc.13746. Epub 2021 Apr 28.
To assess the physiological functions of bound phenols in insoluble dietary fiber (IDF) from different Triticeae crops, bound phenols in IDF were extracted from wheat, triticale, barley and quinoa. In addition, model in vitro was established and used in evaluating the release of bound phenols and changes in their physiological functions after simulated digestion. Results showed that bound phenol content in IDF from Triticeae crops before digestion was higher than which during digestion, and its physiological functions were also better (p < .05). Moreover, barley extracted before digestion, its bound phenolic content in IDF was higher than those in other three Triticeae crops, besides,its bound phenol also showed better antioxidant ability. Before digestion, bound phenols in IDF from triticale showed the best hypoglycemic ability (the inhibition rate of α-glucosidase was 95%; the inhibition rate of α-amylase was 97%). In the simulated digestion model, the bound phenols were mainly released during the intestinal digestion stage, they showed better physiological functions than which released at the gastric digestion stage. The bound phenol content in triticale was higher and its physiological functions was better than those in the other crops. PRACTICAL APPLICATIONS: The results of this experiment showed that the release of bound phenols measured by chemical extraction (i.e., before digestion) was higher than that by simulated gastrointestinal digestion. Compared with the chemical extraction method, in vitro gastro-intestinal digestion simulates the pH value and enzyme environment of food in the human body gastrointestinal digestion process more effectively. This study can provide reference for selecting Triticeae crops feeding in the future. To be more precise, bound phenol content in the insoluble dietary fiber of barley was the highest before digestion. The bound phenol in the insoluble dietary fiber of triticale had the best hypoglycemic ability. The bound phenolic compounds are mainly released during intestinal digestion, and their physiological functions are better than that in gastric digestion.
为了评估来自不同禾本科作物不溶性膳食纤维（IDF）中结合酚的生理功能，从小麦、小黑麦、大麦和藜麦中提取了 IDF 中的结合酚。此外，还建立了体外模型，用于评估结合酚的释放及其在模拟消化后生理功能的变化。结果表明，在消化前，来自禾本科作物的 IDF 中结合酚的含量高于消化过程中的含量，其生理功能也更好（p<0.05）。此外，大麦在消化前提取时，其 IDF 中结合酚的含量高于其他三种禾本科作物，此外，其结合酚也表现出更好的抗氧化能力。在消化前，来自小黑麦的 IDF 中的结合酚表现出最佳的降血糖能力（α-葡萄糖苷酶抑制率为 95%；α-淀粉酶抑制率为 97%）。在模拟消化模型中，结合酚主要在肠道消化阶段释放，其释放后的生理功能优于在胃消化阶段释放的生理功能。小黑麦中的结合酚含量较高，其生理功能优于其他作物。实际应用：本实验结果表明，通过化学提取（即在消化前）测量的结合酚释放量高于通过模拟胃肠消化的释放量。与化学提取方法相比，体外胃肠消化更有效地模拟了食物在人体胃肠道消化过程中的 pH 值和酶环境。本研究可为未来选择禾本科作物饲养提供参考。更准确地说，大麦不溶性膳食纤维中的结合酚在消化前含量最高。小黑麦不溶性膳食纤维中的结合酚具有最佳的降血糖能力。结合酚类化合物主要在肠道消化过程中释放，其生理功能优于胃消化过程中的生理功能。
