College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
Research Institute of Crop Germplasm Resources, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, China.
Food Chem. 2024 Nov 1;457:140124. doi: 10.1016/j.foodchem.2024.140124. Epub 2024 Jun 18.
Phenolics in bound form extensively exist in cereal dietary fiber, especially insoluble fiber, while their release profile in gastrointestinal tract and contribution to the potential positive effects of dietary fiber in modulating gut microbiota still needs to be disclosed. In this work, the composition of bound phenolics (BPs) in triticale insoluble dietary fiber (TIDF) was studied, and in vitro gastrointestinal digestion as well as colonic fermentation were performed to investigate BPs liberation and their role in regulating intestinal flora of TIDF. It turned out that most BPs were unaccessible in digestion but partly released continuously during fermentation. 16 s rRNA sequencing demonstrated that TIDF possessed prebiotic effects by promoting anti-inflammatory while inhibiting proinflammatory bacteria alongside boosting SCFAs production and antioxidative BPs contributed a lot to these effects. Results indicated that TIDF held capabilities to regulate intestinal flora and BPs were important functional components to the health benefits of cereal dietary fiber.
结合态酚广泛存在于谷物膳食纤维中，尤其是不溶性膳食纤维，但其在胃肠道中的释放情况以及对膳食纤维调节肠道菌群潜在积极作用的贡献仍有待揭示。本工作研究了黑小麦不溶性膳食纤维（TIDF）中结合态酚（BPs）的组成，并进行了体外胃肠道消化和结肠发酵实验，以研究 BPs 的释放及其在调节 TIDF 肠道菌群中的作用。结果表明，大多数 BPs 在消化过程中无法被利用，但在发酵过程中会被部分连续释放。16s rRNA 测序结果表明，TIDF 通过促进抗炎菌、抑制促炎菌，同时增加 SCFAs 生成而发挥益生元作用，而抗氧化 BPs 对此也有很大贡献。结果表明，TIDF 具有调节肠道菌群的能力，BPs 是谷物膳食纤维发挥健康益处的重要功能成分。
