PagGRF12a 通过调节 PagXND1a 在 Populus alba × P. glandulosa 中的表达与 PagGIF1b 相互作用，从而调控次生木质部的发育。
PagGRF12a interacts with PagGIF1b to regulate secondary xylem development through modulating PagXND1a expression in Populus alba × P. glandulosa.
机构信息
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Tai'an, 271018, China.
出版信息
J Integr Plant Biol. 2021 Oct;63(10):1683-1694. doi: 10.1111/jipb.13102. Epub 2021 Aug 2.
Growth-regulating factors (GRFs) are important regulators of plant development and growth, but their possible roles in xylem development in woody plants remain unclear. Here, we report that Populus alba × Papulus glandulosa PagGRF12a negatively regulates xylem development in poplar. PagGRF12a is expressed in vascular tissues. Compared to non-transgenic control plants, transgenic poplar plants overexpressing PagGRF12a exhibited reduced xylem width and plants with repressed expression of PagGRF12a exhibited increased xylem width. Xylem NAC domain 1 (XND1) encodes a NAC domain transcription factor that regulates xylem development and transcriptional analyses revealed that PagXND1a is highly upregulated in PagGRF12a-overexpressing plants and downregulated in PagGRF12a-suppressed plants, indicating that PagGRF12a may regulate xylem development through PagXND1a. Transient transcriptional assays and chromatin immunoprecipitation-polymerase chain reaction assays confirmed that PagGRF12a directly upregulates PagXND1a. In addition, PagGRF12a interacts with the GRF-Interacting Factor (GIF) PagGIF1b, and this interaction enhances the effects of PagGRF12a on PagXND1a. Our results indicate that PagGRF12a inhibits xylem development by upregulating the expression of PagXND1a.
生长调节因子（GRFs）是植物发育和生长的重要调节剂，但它们在木本植物木质部发育中的可能作用尚不清楚。在这里，我们报道了白杨 × 腺柳 PagGRF12a 负调控杨树木质部的发育。PagGRF12a 在维管束组织中表达。与非转基因对照植物相比，过表达 PagGRF12a 的转基因杨树植物表现出木质部宽度减小，而 PagGRF12a 表达受抑制的植物表现出木质部宽度增加。木质部 NAC 结构域 1（XND1）编码一个调节木质部发育的 NAC 结构域转录因子，转录分析显示 PagXND1a 在 PagGRF12a 过表达植物中高度上调，在 PagGRF12a 抑制植物中下调，表明 PagGRF12a 可能通过 PagXND1a 调节木质部发育。瞬时转录分析和染色质免疫沉淀聚合酶链反应分析证实 PagGRF12a 直接上调 PagXND1a。此外，PagGRF12a 与 GRF-Interacting Factor（GIF）PagGIF1b 相互作用，这种相互作用增强了 PagGRF12a 对 PagXND1a 的影响。我们的结果表明，PagGRF12a 通过上调 PagXND1a 的表达来抑制木质部的发育。
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