State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China.
Int J Mol Sci. 2024 Mar 31;25(7):3926. doi: 10.3390/ijms25073926.
The discovery of new genes with novel functions is a major driver of adaptive evolutionary innovation in plants. Especially in woody plants, due to genome expansion, new genes evolve to regulate the processes of growth and development. In this study, we characterized the unique transcription factor family in × , which is associated with secondary metabolism. Twenty VeA genes were characterized systematically on their phylogeny, genomic distribution, gene structure and conserved motif, promoter binding site, and expression profiling. Furthermore, through ChIP-qPCR, Y1H, and effector-reporter assays, it was demonstrated that PagMYB128 directly regulated to influence the biosynthesis of secondary metabolites. These results provide a basis for further elucidating the function of gene in poplar and its genetic regulation mechanism.
新基因的发现及其新功能是植物适应性进化创新的主要驱动力。特别是在木本植物中，由于基因组的扩张，新基因进化以调节生长和发育过程。在这项研究中，我们对与次生代谢相关的 × 特有的转录因子家族进行了特征描述。系统地对 20 个 VeA 基因的系统发育、基因组分布、基因结构和保守基序、启动子结合位点和表达谱进行了表征。此外，通过 ChIP-qPCR、Y1H 和效应物报告基因检测，证明 PagMYB128 直接调控 以影响次生代谢物的生物合成。这些结果为进一步阐明杨树中 基因的功能及其遗传调控机制提供了依据。
