生物正交化学能够实现长链RNA寡核苷酸的固相合成以及高效液相色谱和无胶纯化。
Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides.
作者信息
He Muhan, Wu Xunshen, Mao Song, Haruehanroengra Phensinee, Khan Irfan, Sheng Jia, Royzen Maksim
机构信息
University at Albany, Department of Chemistry, Albany, NY,12222, USA.
出版信息
Chem Commun (Camb). 2021 Apr 29;57(35):4263-4266. doi: 10.1039/d1cc00096a.
PMID:33913974
Abstract
Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.
摘要
由于从失败序列中纯化目标链的复杂性，固相合成长度超过100个核苷酸的RNA寡核苷酸仍然具有挑战性。这项工作描述了一种非色谱策略，该策略将使长RNA链的常规固相合成成为可能。
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