Geriatric Unit, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Department of Diabetes, Endocrinology and Nutrition, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
PLoS One. 2021 Apr 29;16(4):e0250856. doi: 10.1371/journal.pone.0250856. eCollection 2021.
Glycolytic metabolism is closely involved in physiological homeostasis and pathophysiological states. Among glycolytic enzymes, phosphoglycerate mutase (PGAM) has been reported to exert certain physiological role in vitro, whereas its impact on glucose metabolism in vivo remains unclear. Here, we report the characterization of Pgam1 knockout mice. We observed that homozygous knockout mice of Pgam1 were embryonic lethal. Although we previously reported that both PGAM-1 and -2 affect global glycolytic profile of cancers in vitro, in vivo glucose parameters were less affected both in the heterozygous knockout of Pgam1 and in Pgam2 transgenic mice. Thus, the impact of PGAM on in vivo glucose metabolism is rather complex than expected before.
糖酵解代谢与生理稳态和病理生理状态密切相关。在糖酵解酶中，磷酸甘油酸变位酶 (PGAM) 已被报道在体外发挥某些生理作用，但其在体内对葡萄糖代谢的影响尚不清楚。在这里，我们报告了 Pgam1 基因敲除小鼠的特征。我们观察到 Pgam1 基因纯合敲除的小鼠胚胎致死。尽管我们之前报道过 PGAM-1 和 -2 都影响体外癌症的整体糖酵解谱，但在 Pgam1 杂合敲除和 Pgam2 转基因小鼠中，体内葡萄糖参数的影响较小。因此，PGAM 对体内葡萄糖代谢的影响比预期的要复杂。
