Laboratório de Química de Produtos Naturais, Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 23897-000, Brasil.
Laboratório de Cromatografia Líquida, Embrapa Agroindústria de Alimentos, Rio de Janeiro, RJ, 23020-470, Brasil.
Chem Biodivers. 2021 Jun;18(6):e2100054. doi: 10.1002/cbdv.202100054. Epub 2021 May 18.
Eugenia copacabanensis and Myrciaria tenella are present in restingas of the Atlantic Forest, but little information is available about their chemical and biological potential. In this context, the hexane, dichloromethane, ethyl acetate and butanol fractions from the leaves of methanolic extract were analyzed by GC/MS and HPLC-DAD and the antioxidant potential was determined by DPPH and ABTS assays and using a Saccharomyces cerevisiae model. Dereplication allowed the identification of 68 compounds, 42 and 41 of which, respectively, are first reported here for E. copacabanensis and M. tenella. In vivo results revealed that the ethyl acetate and butanol fractions showed expressive antioxidant protection in the BY4741 and Δgsh1 strains, with greater impact on glutathione-deficient cells. With a high diversity of phenolic compounds, these polar fractions of E. copacabanensis and M. tenella leaves are potential protectors against intracellular oxidative stress.
长柄胡椒和番樱桃存在于大西洋森林的沿岸沙丘中，但关于它们的化学和生物潜力的信息很少。在这种情况下，对来自甲醇提取物的叶的正己烷、二氯甲烷、乙酸乙酯和正丁醇馏分进行了 GC/MS 和 HPLC-DAD 分析，并通过 DPPH 和 ABTS 测定法以及使用酿酒酵母模型来确定抗氧化潜力。去重允许鉴定 68 种化合物，其中 42 种和 41 种分别首次报道了 E. copacabanensis 和 M. tenella。体内结果表明，乙酸乙酯和正丁醇馏分在 BY4741 和 Δgsh1 菌株中表现出明显的抗氧化保护作用，对谷胱甘肽缺乏的细胞影响更大。这些极性馏分含有丰富的酚类化合物，是长柄胡椒和番樱桃叶中潜在的细胞内氧化应激保护剂。
