da Silva Sá Fabyola Amaral, de Paula Joelma Abadia Marciano, Dos Santos Pierre Alexandre, de Almeida Ribeiro Oliveira Leandra, de Almeida Ribeiro Oliveira Gerlon, Lião Luciano Morais, de Paula José Realino, do Rosário Rodrigues Silva Maria
Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiânia 74605-050, Brazil.
Faculty of Pharmacy, Federal University of Goias, Goiânia 74605-170, Brazil.
Molecules. 2017 Jul 4;22(7):1100. doi: 10.3390/molecules22071100.
This work describes the isolation and structural elucidation of compounds from the leaves of (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fractionate and purify the extract of the leaves and the chemical structures of the compounds were determined using spectroscopic techniques. The antibacterial and antifungal activities were assessed using the broth microdilution method. The phytochemical investigation isolated 11 compounds: α-bisabolol, α-bisabolol oxide B, α-cadinol, β-sitosterol, -pentacosane, -tetracosane, quercetin, kaempferol, avicularin, juglanin and guaijaverin. The crude ethanolic extract and its fractions were tested against 15 bacteria and 9 yeasts. The crude extract inhibited the in vitro growth of yeasts at concentration of 4 to 32 μg/mL. The hexane, dichloromethane, ethyl acetate and aqueous fractions inhibited sp. at concentrations of 4 to 256 μg/mL, whereas the sp. isolates were inhibited only by the hexane and dichloromethane fractions in minimal inhibitory concentrations (MICs) at 16 to 64 μg/mL. The flavonoid quercetin-3--α-arabinofuranose (avicularin) was the most active compound, inhibiting species in concentrations of 2 to 32 μg/mL. The MIC values suggest potential activity of this plant species against yeast.
本研究描述了从（奥布尔）DC.（巴西番樱桃）叶片中分离化合物并阐明其结构，并评估了粗提物、馏分和分离出的化合物对细菌和真菌的抗菌活性。采用柱色谱法对巴西番樱桃叶片提取物进行分离和纯化，并利用光谱技术确定化合物的化学结构。采用肉汤微量稀释法评估抗菌和抗真菌活性。植物化学研究分离出11种化合物：α-红没药醇、α-红没药醇氧化物B、α-杜松醇、β-谷甾醇、二十五烷、二十四烷、槲皮素、山奈酚、扁蓄苷、胡桃苷和桂樱苷。对粗乙醇提取物及其馏分进行了针对15种细菌和9种酵母的测试。粗提取物在浓度为4至32μg/mL时可抑制酵母的体外生长。己烷、二氯甲烷、乙酸乙酯和水馏分在浓度为4至256μg/mL时可抑制酿酒酵母，而白色念珠菌分离株仅在最低抑菌浓度（MIC）为16至64μg/mL时被己烷和二氯甲烷馏分抑制。黄酮类化合物槲皮素-3-α-L-阿拉伯呋喃糖（扁蓄苷）是活性最强的化合物，在浓度为2至32μg/mL时可抑制白色念珠菌。MIC值表明该植物物种对酵母具有潜在活性。
