Department of Biomedical Engineering, The Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran; Department of Biotechnology and Pharmaceutical Engineering, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
Department of Biomedical Engineering, The Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran; Department of Biotechnology and Pharmaceutical Engineering, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
J Mech Behav Biomed Mater. 2021 Jul;119:104511. doi: 10.1016/j.jmbbm.2021.104511. Epub 2021 Apr 22.
Current decellularization methods for articular cartilages require many steps, various and high amounts of detergents, and a relatively long time to produce decellularized scaffolds. In addition, such methods often damage the essential components and the structure of the tissue. This study aims to introduce a novel perfusion-based bioreactor (PBB) method to decellularize bovine articular cartilages efficiently while reducing the harmful physical and chemical steps as well as the duration of the process. This leads to better preservation of the structure and the essential components of the native tissue. Firstly, a certain number of channels (Ø 180 μm) were introduced into both sides of cylindrical articular bovine cartilage disks (5 mm in diameter and 1 mm in thickness). Next, the disks were decellularized in the PBB and a shaker as the control. Using the PBB method resulted in ∼90% reduction of DNA content in the specimens, which was significantly higher than those of the shaker results with ∼60%. Also, ∼50% sulfated glycosaminoglycan (sGAG) content and ∼92% of the compression properties were maintained implying the efficient preservation of the structure and components of the scaffolds. Moreover, the current study indicated that the PBB specimens supported the adherence and proliferation of the new cells effectively. In conclusion, the results show that the use of PBB method increases the efficiency of producing decellularized cartilage scaffolds with a better maintenance of essential components and structure, while reducing the chemicals and steps required for the process. This will pave the way for producing close-to-natural scaffolds for cartilage tissue engineering.
目前用于关节软骨的脱细胞方法需要经过许多步骤、使用各种高浓度的去污剂，并需要相对较长的时间来制备脱细胞支架。此外，这种方法往往会破坏组织的重要成分和结构。本研究旨在介绍一种新的灌注式生物反应器（PBB）方法，以有效脱细胞化牛关节软骨，同时减少有害的物理和化学步骤以及过程的持续时间。这可以更好地保存组织和天然组织的重要成分。首先，在圆柱形关节牛软骨盘的两侧引入一定数量的通道（Ø 180 µm）（直径 5mm，厚度 1mm）。然后，将圆盘在 PBB 和摇床中进行脱细胞处理作为对照。使用 PBB 方法可使标本中的 DNA 含量减少约 90%，明显高于摇床结果的约 60%。此外，还保持了约 50%的硫酸化糖胺聚糖（sGAG）含量和约 92%的压缩性能，这意味着支架的结构和成分得到了有效保存。此外，本研究表明，PBB 标本能够有效地支持新细胞的黏附和增殖。总之，结果表明，使用 PBB 方法可以提高脱细胞软骨支架的制备效率，同时更好地保持重要成分和结构，减少所需的化学物质和步骤。这将为软骨组织工程制备更接近天然的支架铺平道路。
