Pazdzior Robert, Kubicek Stefan
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
HardwareX. 2021 Aug 11;10:e00222. doi: 10.1016/j.ohx.2021.e00222. eCollection 2021 Oct.
Here we present a versatile system for milliliter-scale perfusion culture of adherent cells that can be built using basic tools, based on a readily available one-well culture plate (84 cm culture area). Media composition and flow paths can be programmatically controlled via USB serial interface using the FETbox hardware controller and associated PlateFlo Python package. The FETbox can control up to five high current 12 V devices such as common pinch valves, solenoids, and DC motor peristaltic pumps. It was designed to be easily customized with built-in accommodation for additional electronic components (e.g. analog sensors and input), use of the ubiquitous Arduino Nano platform, and easily expanded serial communication protocol. Multiple FETboxes can be used in parallel for additional devices. Applications of the PlateFlo system include perfusion culture of laboratory experiments requiring large cell numbers including genome-scale genetic screens and proteomics, as well as novel perfusion schemes including dynamic media conditions and sequential cell culture.
在此，我们展示了一种用于贴壁细胞毫升规模灌注培养的通用系统，该系统可使用基本工具构建，基于一种现成的单孔培养板（84平方厘米培养面积）。培养基成分和流动路径可通过USB串行接口，使用FETbox硬件控制器和相关的PlateFlo Python软件包进行编程控制。FETbox最多可控制五个高电流12V设备，如普通夹管阀、电磁阀和直流电机蠕动泵。它的设计易于定制，内置了对额外电子元件（如模拟传感器和输入）的容纳空间，采用了普遍使用的Arduino Nano平台，并具有易于扩展的串行通信协议。多个FETbox可并行用于额外的设备。PlateFlo系统的应用包括对需要大量细胞的实验室实验进行灌注培养，包括基因组规模的遗传筛选和蛋白质组学，以及包括动态培养基条件和顺序细胞培养在内的新型灌注方案。
