Chen Yifei, Zhang Chenyue, Zhang Shiyong, Qi Hexu, Zhang Donghui, Li Yifei, Fang Jie
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, China.
Front Bioeng Biotechnol. 2022 Aug 22;10:987999. doi: 10.3389/fbioe.2022.987999. eCollection 2022.
Artificial articular cartilage (AC) is extensively applied in the repair and regeneration of cartilage which lacks self-regeneration capacity because of its avascular and low-cellularity nature. With advances in tissue engineering, bioengineering techniques for artificial AC construction have been increasing and maturing gradually. In this review, we elaborated on the advances of biological scaffold technologies in artificial AC including freeze-drying, electrospinning, 3D bioprinting and decellularized, and scaffold-free methods such as self-assembly and cell sheet. In the following, several successful applications of artificial AC built by scaffold and scaffold-free techniques are introduced to demonstrate the clinical application value of artificial AC.
人工关节软骨（AC）因其无血管和低细胞性的特性而缺乏自我再生能力，因此被广泛应用于软骨的修复和再生。随着组织工程学的发展，用于构建人工AC的生物工程技术不断增加并逐渐成熟。在本综述中，我们阐述了人工AC中生物支架技术的进展，包括冷冻干燥、静电纺丝、3D生物打印和脱细胞技术，以及无支架方法，如自组装和细胞片技术。接下来，介绍了几种通过支架和无支架技术构建的人工AC的成功应用，以证明人工AC的临床应用价值。
