Bidi Hossein, Fallah Hormoz, Niknejad Yosoof, Barari Tari Davood
Department of Agronomy, Islamic Azad University of Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Department of Agronomy, Islamic Azad University of Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Plant Physiol Biochem. 2021 Jun;163:348-357. doi: 10.1016/j.plaphy.2021.04.020. Epub 2021 Apr 23.
The food chain contaminated with arsenic (As) has developed a hazardous threat to the growth and development of plants, animals and humans. The present study was conducted to examine the application of iron oxide nanoparticles (FeNPs) on biochemical and molecular traits of roots and leaves of rice plants under As phytotoxicity. The results showed that As reduced the accumulation of Fe in roots and leaves and thus reduced photosynthetic pigments and growth of rice plants. As stress enhanced the accumulation of hydrogen peroxide, superoxide anion and methylglyoxal by increasing the accumulation of As in roots and leaves, resulting in damage to membrane lipids and raised electrolyte leakage (EL). However, FeNPs strengthen the glyoxalase system and antioxidant enzymes, thereby alleviating oxidative stress and reducing EL. FeNPs protected plant cells from As phytotoxicity by enhancing the accumulation of chelating agents (proline, glutathione and phytochelatins) and the sequestration and immobilization of As in the vacuoles and the cell walls. FeNPs downregulated the expression of genes involved in As uptake and translocation (Lsi1 and Lsi2) and, consequently, reduced As accumulation in the roots and leaves of As-stressed plants. FeNPs also improved the accumulation of Fe in the roots and leaves by modulating the expression of genes that regulate Fe uptake and its transport to leaves (IRT1, IRT2, YSL2, YSL13, FRDL1, DMAS1, NAS2 and NAS3), resulting in the restoration of photosynthetic pigments and the growth of As-stressed plants. Our findings authenticate the role of FeNPs in diminishing As phytotoxicity on rice.
被砷（As）污染的食物链已对植物、动物和人类的生长发育构成了有害威胁。本研究旨在考察氧化铁纳米颗粒（FeNPs）对受砷毒害的水稻植株根和叶的生化及分子特性的影响。结果表明，砷降低了根和叶中铁的积累，从而减少了光合色素含量并抑制了水稻植株的生长。砷胁迫通过增加根和叶中砷的积累，增强了过氧化氢、超氧阴离子和甲基乙二醛的积累，导致膜脂损伤并提高了电解质渗漏率（EL）。然而，FeNPs增强了乙二醛酶系统和抗氧化酶，从而减轻了氧化应激并降低了EL。FeNPs通过增强螯合剂（脯氨酸、谷胱甘肽和植物螯合肽）的积累以及将砷隔离和固定在液泡和细胞壁中，保护植物细胞免受砷的毒害。FeNPs下调了参与砷吸收和转运的基因（Lsi1和Lsi2）的表达，因此减少了受砷胁迫植株根和叶中的砷积累。FeNPs还通过调节调控铁吸收及其向叶运输的基因（IRT1、IRT2、YSL2、YSL13、FRDL1、DMAS1、NAS2和NAS3）的表达，提高了根和叶中铁的积累，从而恢复了受砷胁迫植株的光合色素含量和生长。我们的研究结果证实了FeNPs在减轻砷对水稻的毒害作用方面的作用。
