Shaghaleh Hiba, Alhaj Hamoud Yousef, Saleem Muhammad Hamzah, Elansary Hosam O, Mahmoud Eman A, Sheteiwy Mohamed S
School of Energy and Environment, Southeast University, Nanjing, 210096, China.
College of Hydrology and Water Recourses, Hohai University, Nanjing, 210024, China.
Sci Rep. 2025 Jul 24;15(1):26953. doi: 10.1038/s41598-025-11629-z.
Utilizing phytohormones-brassinosteroids (BRs), jasmonic acid (JA), and strigolactones (SLs) presents a novel approach to overcome toxicity of arsenic (As) in agricultural settings. The present study investigates the role of three phytohormones BRs, JA, and SLs in ameliorating As-induced stressed in Oryza sativa L. (rice) seedlings. s In the present study, we have used different levels of BRs (0.1 µM), JA (1 µM), and SLs (0.05 µM) every 7 days to O. sativa seedlings when exposed to different levels of As levels of 100 mg kg and 200 mg kg (as sodium arsenate) to examine various growth and biochemical parameters in O. sativa. The results showed that the As toxicity in the soil showed a significantly (P < 0.05) declined in the growth, gas exchange attributes, sugars, AsA-GSH cycle, cellular fractionation, proline metabolism in O. sativa. However, As toxicity significantly (P < 0.05) increased oxidative stress biomarkers, enzymatic and nonenzymatic antioxidants including their gene expression in O. sativa. Although, the application of phytohormones including BRs, JA, and SLs showed a significant (P < 0.05) increase in the plant growth and biomass, gas exchange characteristics, enzymatic and non-enzymatic compounds and their gene expression and also decreased the oxidative stress, and As uptake. In addition, individual or combined application of phytohormones including BRs, JA, and SLs enhanced the cellular fractionation and decreases the proline metabolism and AsA - GSH cycle in O. sativa. These results open new insights for sustainable agriculture practices and hold immense promise in addressing the pressing challenges of heavy metal contamination in agricultural soils.
利用植物激素——油菜素甾醇（BRs）、茉莉酸（JA）和独脚金内酯（SLs），为在农业环境中克服砷（As）的毒性提供了一种新方法。本研究调查了三种植物激素BRs、JA和SLs在缓解水稻幼苗砷胁迫中的作用。在本研究中，当水稻幼苗暴露于100 mg/kg和200 mg/kg（以砷酸钠形式）的不同水平砷时，我们每隔7天使用不同水平的BRs（0.1 µM）、JA（1 µM）和SLs（0.05 µM），以检测水稻的各种生长和生化参数。结果表明，土壤中的砷毒性使水稻的生长、气体交换属性、糖类、AsA-GSH循环、细胞分级分离、脯氨酸代谢显著（P<0.05）下降。然而，砷毒性显著（P<0.05）增加了水稻中的氧化应激生物标志物、酶促和非酶促抗氧化剂，包括它们的基因表达。尽管如此，施用包括BRs、JA和SLs在内的植物激素，使植物生长和生物量、气体交换特征、酶促和非酶促化合物及其基因表达显著（P<0.05）增加，同时降低了氧化应激和砷吸收。此外，包括BRs、JA和SLs在内的植物激素单独或联合施用，增强了水稻的细胞分级分离，并降低了脯氨酸代谢和AsA-GSH循环。这些结果为可持续农业实践开辟了新的思路，并在应对农业土壤重金属污染的紧迫挑战方面具有巨大潜力。
