• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

生理浓度的维生素C对体外抑制Fe-EGTA-H₂O和Fe-EGTA-H₂O₂体系中羟基自由基诱导发光的影响。

Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe-EGTA-HO and Fe-EGTA-HO Systems In Vitro.

作者信息

Nowak Michal, Tryniszewski Wieslaw, Sarniak Agata, Wlodarczyk Anna, Nowak Piotr J, Nowak Dariusz

机构信息

Radiation Protection, University Hospital No. 2, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland.

Department of Radiological and Isotopic Diagnostics and Therapy, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland.

出版信息

Molecules. 2021 Apr 1;26(7):1993. doi: 10.3390/molecules26071993.

DOI:10.3390/molecules26071993
PMID:33915907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8037725/
Abstract

Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe/Fe ions and HO, it may behave as a pro-oxidant by accelerating and enhancing the formation of hydroxyl radicals (OH). Therefore, in this study we evaluated the effect of AA at concentrations of 1 to 200 µmol/L on OH-induced light emission (at a pH of 7.4 and temperature of 37 °C) from 92.6 µmol/L Fe-185.2 µmol/L EGTA (ethylene glycol-bis (β-aminoethyl ether)-N,N,N',N'-tetraacetic acid)-2.6 mmol/L HO, and 92.6 µmol/L Fe-185.2 µmol/L EGTA-2.6 mmol/L HO systems. Dehydroascorbic acid (DHAA) at the same range of concentrations served as the reference compound. Light emission was measured with multitube luminometer (AutoLumat Plus LB 953) for 120 s after automatic injection of HO. AA at concentrations of 1 to 50 µmol/L and of 1 to 75 µmol/L completely inhibited light emission from Fe-EGTA-HO and Fe-EGTA-HO, respectively. Concentrations of 100 and 200 µmol/L did not affect chemiluminescence of Fe-EGTA-HO but tended to increase light emission from Fe-EGTA-HO. DHAA at concentrations of 1 to 100 µmol/L had no effect on chemiluminescence of both systems. These results indicate that AA at physiological concentrations exhibits strong antioxidant activity in the presence of chelated iron and HO.

摘要

抗坏血酸（AA）具有抗氧化特性。然而，在铁离子/亚铁离子和过氧化氢存在的情况下，它可能通过加速和增强羟基自由基（·OH）的形成而表现为促氧化剂。因此，在本研究中，我们评估了浓度为1至200μmol/L的AA对92.6μmol/L铁 - 185.2μmol/L乙二醇双（β - 氨基乙醚）-N，N，N'，N'-四乙酸（EGTA）-2.6 mmol/L过氧化氢以及92.6μmol/L铁 - 185.2μmol/L EGTA - 2.6 mmol/L过氧化氢体系中·OH诱导的发光（在pH 7.4和37℃温度下）的影响。相同浓度范围的脱氢抗坏血酸（DHAA）用作参考化合物。在自动注入过氧化氢后，使用多管发光计（AutoLumat Plus LB 953）测量120秒的发光情况。浓度为1至50μmol/L的AA和1至75μmol/L的AA分别完全抑制了铁 - EGTA - 过氧化氢和铁 - EGTA - 过氧化氢体系的发光。100和200μmol/L的浓度对铁 - EGTA - 过氧化氢的化学发光没有影响，但倾向于增加铁 - EGTA - 过氧化氢体系的发光。浓度为1至100μmol/L的DHAA对两个体系的化学发光均无影响。这些结果表明，在生理浓度下，AA在螯合铁和过氧化氢存在的情况下表现出强大抗氧化活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/cedaa4b0c571/molecules-26-01993-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/fb964e2d8fd3/molecules-26-01993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/a3efb54d3966/molecules-26-01993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/f887c062a59d/molecules-26-01993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/3fc36292d49f/molecules-26-01993-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/cedaa4b0c571/molecules-26-01993-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/fb964e2d8fd3/molecules-26-01993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/a3efb54d3966/molecules-26-01993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/f887c062a59d/molecules-26-01993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/3fc36292d49f/molecules-26-01993-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdfd/8037725/cedaa4b0c571/molecules-26-01993-g005.jpg

相似文献

1
Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe-EGTA-HO and Fe-EGTA-HO Systems In Vitro.生理浓度的维生素C对体外抑制Fe-EGTA-H₂O和Fe-EGTA-H₂O₂体系中羟基自由基诱导发光的影响。
Molecules. 2021 Apr 1;26(7):1993. doi: 10.3390/molecules26071993.
2
Light Emission from the Fe-EGTA-H₂O₂ System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics.Fe-EGTA-H₂O₂ 体系的发光：用于测定植物酚类抗氧化活性的可能应用。
Molecules. 2018 Apr 10;23(4):866. doi: 10.3390/molecules23040866.
3
Light Emission from Fe-EGTA-HO System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body.Fe-EGTA-HO 体系的发光取决于反应介质的 pH 值，该 pH 值范围可能出现在人体细胞中。
Molecules. 2024 Aug 25;29(17):4014. doi: 10.3390/molecules29174014.
4
Concentration Dependence of Anti- and Pro-Oxidant Activity of Polyphenols as Evaluated with a Light-Emitting Fe-Egta-HO System.用发光 Fe-Egta-HO 体系评估多酚的抗氧化和促氧化剂活性的浓度依赖性。
Molecules. 2022 May 27;27(11):3453. doi: 10.3390/molecules27113453.
5
Light emission from the Fe -EDTA-ascorbic acid-H O system strongly enhanced by plant phenolic acids.植物酚酸能强烈增强Fe -EDTA-抗坏血酸-H₂O体系的发光。
Luminescence. 2019 Mar;34(2):183-192. doi: 10.1002/bio.3591. Epub 2019 Jan 24.
6
Hydroxyl radical generation by a light-dependent Fenton reaction.通过光依赖性芬顿反应产生羟基自由基。
Free Radic Biol Med. 1993 Feb;14(2):105-13. doi: 10.1016/0891-5849(93)90001-b.
7
Antioxidant properties of carnosine re-evaluated with oxidizing systems involving iron and copper ions.通过涉及铁离子和铜离子的氧化系统对肌肽的抗氧化特性进行重新评估。
Basic Clin Pharmacol Toxicol. 2005 May;96(5):352-60. doi: 10.1111/j.1742-7843.2005.pto_03.x.
8
Polyphenol tannic acid inhibits hydroxyl radical formation from Fenton reaction by complexing ferrous ions.多酚鞣酸通过络合亚铁离子抑制芬顿反应中羟基自由基的形成。
Biochim Biophys Acta. 1999 Oct 18;1472(1-2):142-52. doi: 10.1016/s0304-4165(99)00117-8.
9
The hydrolysis product of ICRF-187 promotes iron-catalysed hydroxyl radical production via the Fenton reaction.ICRF-187的水解产物通过芬顿反应促进铁催化的羟基自由基生成。
Biochem Pharmacol. 1993 May 25;45(10):1967-72. doi: 10.1016/0006-2952(93)90005-h.
10
Identifying the reactive sites of hydrogen peroxide decomposition and hydroxyl radical formation on chrysotile asbestos surfaces.鉴定在温石棉表面上过氧化氢分解和羟基自由基形成的活性部位。
Part Fibre Toxicol. 2020 Jan 20;17(1):3. doi: 10.1186/s12989-019-0333-1.

引用本文的文献

1
Ultra-Weak Photon Emission from Crown Ethers Exposed to Fenton's Reagent Fe-HO.暴露于芬顿试剂Fe-HO的冠醚的超微弱光子发射。
Molecules. 2025 Aug 5;30(15):3282. doi: 10.3390/molecules30153282.
2
Role of micronutrition in patients with oral cancer and nutritional intervention strategies.微量营养素在口腔癌患者中的作用及营养干预策略
Front Nutr. 2025 Jul 24;12:1616344. doi: 10.3389/fnut.2025.1616344. eCollection 2025.
3
Potential Health Impacts, Treatments, and Countermeasures of Martian Dust on Future Human Space Exploration.

本文引用的文献

1
Trying to Solve the Puzzle of the Interaction of Ascorbic Acid and Iron: Redox, Chelation and Therapeutic Implications.试图解开抗坏血酸与铁相互作用之谜：氧化还原、螯合作用及治疗意义
Medicines (Basel). 2020 Jul 30;7(8):45. doi: 10.3390/medicines7080045.
2
Ascorbic acid: The chemistry underlying its antioxidant properties.抗坏血酸：其抗氧化性能的化学基础。
Free Radic Biol Med. 2020 Nov 1;159:37-43. doi: 10.1016/j.freeradbiomed.2020.07.013. Epub 2020 Jul 30.
3
Redox Interactions of Vitamin C and Iron: Inhibition of the Pro-Oxidant Activity by Deferiprone.
火星尘埃对未来人类太空探索的潜在健康影响、治疗方法及应对措施
Geohealth. 2025 Feb 12;9(2):e2024GH001213. doi: 10.1029/2024GH001213. eCollection 2025 Feb.
4
Effect of Selected Organic Solvents on Hydroxyl Radical-Dependent Light Emission in the Fe-EGTA-HO System.选定有机溶剂对Fe-EGTA-H₂O体系中羟基自由基依赖性发光的影响
Molecules. 2024 Nov 28;29(23):5635. doi: 10.3390/molecules29235635.
5
Light Emission from Fe-EGTA-HO System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body.Fe-EGTA-HO 体系的发光取决于反应介质的 pH 值，该 pH 值范围可能出现在人体细胞中。
Molecules. 2024 Aug 25;29(17):4014. doi: 10.3390/molecules29174014.
6
Concentration Dependence of Anti- and Pro-Oxidant Activity of Polyphenols as Evaluated with a Light-Emitting Fe-Egta-HO System.用发光 Fe-Egta-HO 体系评估多酚的抗氧化和促氧化剂活性的浓度依赖性。
Molecules. 2022 May 27;27(11):3453. doi: 10.3390/molecules27113453.
维生素 C 和铁的氧化还原相互作用：去铁酮抑制其促氧化剂活性。
Int J Mol Sci. 2020 May 31;21(11):3967. doi: 10.3390/ijms21113967.
4
Low-molecular-mass iron complexes in blood plasma of iron-deficient pigs do not originate directly from nutrient iron.缺铁猪血浆中的低分子质量铁配合物并非直接来源于营养铁。
Metallomics. 2019 Nov 1;11(11):1900-1911. doi: 10.1039/c9mt00152b. Epub 2019 Oct 11.
5
Degradation of benzoic acid in an advanced oxidation process: The effects of reducing agents.在高级氧化过程中苯甲酸的降解：还原剂的影响。
J Hazard Mater. 2020 Jan 15;382:121090. doi: 10.1016/j.jhazmat.2019.121090. Epub 2019 Aug 24.
6
Kinetic Evaluation of Dye Decolorization by Fenton Processes in the Presence of 3-Hydroxyanthranilic Acid.在 3-羟基邻氨基苯甲酸存在下通过芬顿工艺对染料进行脱色的动力学评价。
Int J Environ Res Public Health. 2019 May 7;16(9):1602. doi: 10.3390/ijerph16091602.
7
Partial pressure of oxygen in the human body: a general review.人体中的氧分压：综述
Am J Blood Res. 2019 Feb 15;9(1):1-14. eCollection 2019.
8
Unravelling the Interplay between Extracellular Acidosis and Immune Cells.解析细胞外酸中毒与免疫细胞间的相互作用。
Mediators Inflamm. 2018 Dec 30;2018:1218297. doi: 10.1155/2018/1218297. eCollection 2018.
9
The oxidation of dehydroascorbic acid and 2,3-diketogulonate by distinct reactive oxygen species.不同活性氧物质对脱氢抗坏血酸和 2,3-二酮古洛糖酸的氧化作用。
Biochem J. 2018 Nov 9;475(21):3451-3470. doi: 10.1042/BCJ20180688.
10
Light Emission from the Fe-EGTA-H₂O₂ System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics.Fe-EGTA-H₂O₂ 体系的发光：用于测定植物酚类抗氧化活性的可能应用。
Molecules. 2018 Apr 10;23(4):866. doi: 10.3390/molecules23040866.