Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba 305-8572, Japan.
Int J Mol Sci. 2021 Apr 5;22(7):3768. doi: 10.3390/ijms22073768.
Cyanidin, a kind of anthocyanin, has been reported to have chemotherapeutic activities in humans. Human amniotic epithelial cells (hAECs) are considered a potential source of pluripotent stem cells. hAECs have been used as a novel tool in regenerative cellular therapy and cell differentiation studies. In this study, to explore the effects of cyanidin-3--glucoside (Cy3G) on hAECs and their mechanisms, we investigated the transcriptomic changes in the Cy3G-treated cells using microarray analysis. Among the differentially expressed genes (Fold change > 1.1; -value < 0.05), 109 genes were upregulated and 232 were downregulated. Ratios of upregulated and downregulated genes were 0.22% and 0.47% of the total expressed genes, respectively. Next, we explored the enriched gene ontology, i.e., the biological process, molecular function, and cellular component of the 37 upregulated (>1.3-fold change) and 124 downregulated (<1.3-fold change) genes. Significantly enriched biological processes by the upregulated genes included "response to muscle activity," and the genes involved in this gene ontology (GO) were and , which function in the adipocyte. On the other hand, the cell cycle biological process was significantly enriched by the downregulated genes, including some from the gene family. An adipogenesis-associated gene was also included in the cell cycle biological process. Thus, our findings suggest the prospects of Cy3G in modulating adipocyte differentiation in hAECs.
矢车菊素是一种花色苷，已被报道在人类中具有化学治疗活性。人羊膜上皮细胞（hAECs）被认为是多能干细胞的潜在来源。hAECs 已被用作再生细胞治疗和细胞分化研究的新型工具。在这项研究中，为了探索矢车菊素-3-O-葡萄糖苷（Cy3G）对 hAECs 的影响及其机制，我们使用微阵列分析研究了 Cy3G 处理细胞的转录组变化。在差异表达基因中（倍数变化>1.1；-值<0.05），有 109 个基因上调，232 个基因下调。上调和下调基因的比例分别为总表达基因的 0.22%和 0.47%。接下来，我们探讨了上调的 37 个（>1.3 倍变化）和下调的 124 个基因（<1.3 倍变化）的基因本体论（GO）的富集，即生物学过程、分子功能和细胞组成。上调基因显著富集的生物学过程包括“对肌肉活动的反应”，参与该 GO 的基因包括 和 ，它们在脂肪细胞中发挥作用。另一方面，下调基因显著富集细胞周期生物学过程，包括一些来自 基因家族的基因。与脂肪生成相关的基因 也包括在细胞周期生物学过程中。因此，我们的研究结果表明 Cy3G 有望调节 hAECs 中的脂肪细胞分化。
