QuEChERS LC-MS/MS 法检测谷物制品和香料中的真菌毒素
QuEChERS LC-MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices.
机构信息
Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy.
Dipartimento di Scienze della Vita, Università degli studi di Modena e Reggio Emilia, Via Università 4, 41121 Modena, Italy.
出版信息
Int J Environ Res Public Health. 2021 Apr 4;18(7):3774. doi: 10.3390/ijerph18073774.
We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB, AFB, AFG, AFG), deoxynivalenol (DON), fumonisin (FUMO; FB, FB, FB), T2, and HT2 were validated in maize. AFLA and DON were validated in black pepper. The method satisfies the requirements of Commission Regulation (EC) no. 401/2006 and (EC) no. 1881/2006. The screening target concentration (STC) was under maximum permitted levels (MLs) for all mycotoxins validated. The method's performance was assessed by two different proficiencies and tested with 100 real samples.
我们开发并验证了一种用于谷物产品和香料中霉菌毒素分析的筛选方法。该方法采用超高效液相色谱-串联质谱联用技术（UHPLC-MS/MS）进行分析。采用分散固相萃取（d-SPE）进行样品提取。在玉米中验证了赭曲霉毒素 A（OTA）、玉米赤霉烯酮（ZEA）、黄曲霉毒素（AFLA；AFB₁、AFB₂、AFG₁、AFG₂）、脱氧雪腐镰刀菌烯醇（DON）、伏马菌素（FUMO；FB₁、FB₂、FB₃）、T2 和 HT2。在黑胡椒中验证了黄曲霉毒素 A 和 DON。该方法符合欧盟委员会法规（EC）No.401/2006 和（EC）No.1881/2006 的要求。所有验证的霉菌毒素的筛选目标浓度（STC）均低于最大允许水平（MLs）。该方法的性能通过两个不同的能力验证进行评估，并使用 100 个实际样品进行了测试。
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