School of Pharmacy, Shujitsu University, Nishigawara, Okayama 703-8516, Japan.
Molecules. 2021 Apr 3;26(7):2056. doi: 10.3390/molecules26072056.
Active and passive smoking are serious public health concerns Assessment of tobacco smoke exposure using effective biomarkers is needed. In this study, we developed a simultaneous determination method of five tobacco-specific nitrosamines (TSNAs) in hair by online in-tube solid-phase microextraction (SPME) coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS). TSNAs were extracted and concentrated on Supel-Q PLOT capillary by in-tube SPME and separated and detected within 5 min by LC-MS/MS using Capcell Pak C18 MGIII column and positive ion mode multiple reaction monitoring systems. These operations were fully automated by an online program. The calibration curves of TSNAs showed good linearity in the range of 0.5-1000 pg mL using their stable isotope-labeled internal standards. Moreover, the limits of detection ( = 3) of TSNAs were in the range of 0.02-1.14 pg mL, and intra-day and inter-day precisions were below 7.3% and 9.2% ( = 5), respectively. The developed method is highly sensitive and specific and can easily measure TSNA levels using 5 mg hair samples. This method was used to assess long-term exposure levels to tobacco smoke in smokers and non-smokers.
主动和被动吸烟是严重的公共卫生问题，需要使用有效的生物标志物来评估烟草烟雾暴露。在这项研究中，我们开发了一种通过在线管内固相微萃取（SPME）与液相色谱-串联质谱（LC-MS/MS）联用同时测定头发中五种烟草特异性亚硝胺（TSNAs）的方法。TSNAs 通过管内 SPME 被提取和浓缩在 Supel-Q PLOT 毛细管上，并通过 Capcell Pak C18 MGIII 柱和正离子模式多反应监测系统在 5 分钟内被 LC-MS/MS 分离和检测。这些操作通过在线程序全自动完成。TSNAs 的校准曲线在使用其稳定同位素标记内标时，在 0.5-1000 pg/mL 的范围内呈现出良好的线性。此外，TSNAs 的检测限（ = 3）在 0.02-1.14 pg/mL 的范围内，日内和日间精密度分别低于 7.3%和 9.2%（ = 5）。所开发的方法具有很高的灵敏度和特异性，可以使用 5mg 头发样本轻松测量 TSNA 水平。该方法用于评估吸烟者和非吸烟者长期暴露于烟草烟雾的水平。
